Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in response to oxidative stress remains largely unknown. In this study, transcriptomic profiling of glyphosate-tolerant Enterobacter strain NRS-1 was analyzed under 9 mM H₂O₂ stress using RNA-seq and qRT-PCR. The lag period in the growth of NRS-1 was very short compared with wild-type strain under H₂O₂ treatment. A total of 113 genes are identified as differentially expressed genes (DEGs) under H₂O₂ that include 38 upregulated and 75 downregulated transcripts. But not any genes regulated by major oxidative regulons, viz., oxyR, soxR, rpoS, perR, ohrR, and σ^в, have been reported in DEGs, hence potentially reflecting that specific changes have occurred in NRS-1 for adaptation to oxidative stress. Based on the functions of the DEGs, six elements namely formate dehydrogenase, processes associated with iron ions, repair programs, multidrug resistance, antioxidant defense, and energy generation (mqo, sdhC) might have contributed for stress tolerance in NRS-1. These elements are proposed to form a molecular network explaining gene response of NRS-1 to stress, and ensure global cell protection and growth recovery of NRS-1. These findings enrich the view of gene regulation in bacteria in response to H₂O₂ oxidative stress.

自然界中的微生物经常会遭受氧化应激，严重影响其生长速度和生存能力。尽管已经在化学，生理和分子水平上表征了微生物对氧化应激的反应，但是细菌对氧化应激的基因调控网络适应机制仍是很大程度上未知的。在这项研究中，耐草甘膦肠杆菌菌株NRS-1的转录组谱分析在9 mM H ₂ O ₂胁迫下使用RNA-seq和qRT-PCR分析。H ₂ O ₂下与野生型菌株相比，NRS-1生长的滞后期非常短治疗。总共113个基因被鉴定为H ₂ O ₂下的差异表达基因（DEG），其中包括38个上调的转录本和75个下调的转录本。但不是主要的氧化调节子，即规定的任何基因，oxyR，soxR，的rpoS，PERR，ohrR和σ ^в，已经报道了度的视角，因此可能反映了特定的变化已经在发生NRS-1以适应氧化应激。根据DEG的功能，六种元素即甲酸脱氢酶，与铁离子有关的过程，修复程序，多药耐药性，抗氧化防御和能量产生（mqo，sdhC）可能对NRS-1的抗逆性有所帮助。这些元件提出以形成分子网络解释基因响应NRS-1到应力，并确保全局小区保护和生长恢复NRS-1 。这些发现丰富了响应H ₂ O ₂氧化应激的细菌基因调控的观点。