The integration of structure and function for tissue engineering scaffolds is of great importance in mimicking native bone tissue. However, the complexity of hierarchical structures, the requirement for mechanical properties, and the diversity of bone resident cells are the major challenges in constructing biomimetic bone tissue engineering scaffolds. Herein, a Haversian bone–mimicking scaffold with integrated hierarchical Haversian bone structure was successfully prepared via digital laser processing (DLP)–based 3D printing. The compressive strength and porosity of scaffolds could be well controlled by altering the parameters of the Haversian bone–mimicking structure. The Haversian bone–mimicking scaffolds showed great potential for multicellular delivery by inducing osteogenic, angiogenic, and neurogenic differentiation in vitro and accelerated the ingrowth of blood vessels and new bone formation in vivo. The work offers a new strategy for designing structured and functionalized biomaterials through mimicking native complex bone tissue for tissue regeneration.

组织工程支架的结构和功能的整合在模仿天然骨组织中非常重要。然而，分层结构的复杂性，对机械性能的要求以及骨驻留细胞的多样性是构建仿生骨组织工程支架的主要挑战。在此，通过基于数字激光加工（DLP）的3D打印成功地制备了具有集成的分级Haversian骨骼结构的Haversian模仿骨骼的支架。通过改变哈弗斯仿骨结构的参数，可以很好地控制脚手架的抗压强度和孔隙率。Haversian拟骨支架通过诱导成骨，血管生成，体外神经源性分化，并在体内加速血管向内生长和新骨形成。这项工作提供了一种新的策略，可以通过模仿天然的复杂骨骼组织来组织再生来设计结构化和功能化的生物材料。