Strain Z195 was isolated and identified as Paracoccus denitrificans. Z195 exhibited efficient aerobic denitrification and carbon removal abilities, and removed 93.74% of total nitrogen (TN) and 97.81% of total organic carbon.71.88% of nitrogen was lost as gaseous products.¹³C-metabolic flux analysis revealed that 95% and 132% of the carbon fluxes entered the Entner-Doudoroff (ED) pathway and tricarboxylic acid (TCA) cycle, respectively. Electrons produced by carbon metabolism markedly promoted the processes of nitrogen metabolism process and aerobic respiration. A response surface methodology model demonstrated that the optimal conditions for the maximum TN removal were a C/N ratio of 7.47, shaking speed of 108 rpm, temperature of 31°C and initial pH of 8.02. Additionally, the average TN and chemical oxygen demand removal efficiencies of raw wastewater were 89% and 91%, respectively. The results give new insight for understanding metabolic flux analysis of aerobic denitrifying bacteria.

菌株Z195被分离并鉴定为脱氮副球菌。Z195表现出有效的好氧反硝化和除碳能力，去除了总氮（TN）的93.74％和总有机碳的97.81％。气态产物损失了71.88％的氮。¹³C代谢通量分析表明，分别有95％和132％的碳通量进入Entner-Doudoroff（ED）途径和三羧酸（TCA）循环。碳代谢产生的电子显着促进了氮代谢过程和有氧呼吸过程。响应表面方法模型表明，最大去除TN的最佳条件是C / N比为7.47，摇动速度为108 rpm，温度为31°C和初始pH为8.02。此外，原废水的总氮和化学需氧量去除效率分别为89％和91％。结果为了解好氧反硝化细菌的代谢通量分析提供了新的见识。