The aim of this paper was the development of a method for the determination of six strobilurins (fungicides) using boron-doped diamond (BDD) electrode with amperometric detection (AD) homemade coupled to high performance liquid chromatography (HPLC/UV–Vis). HPLC separation of fungicides was performed in a C18 reverse phase column using both UV and AD detectors at 200 mn and 1.9 V, respectively. The linear range for each strobilurin was from 5 to 15 mg L⁻¹ and the correlation coefficients for all the compounds were above 0.997. Both detectors presented adequate detectability (LOD ranging from 1.33 to 1.57 μg kg⁻¹) respecting the limits pre-established by regulatory agencies. The method was validated presenting good values of recovery and accuracy. In the spiked samples the recoveries ranged from 61.6% (trifloxystrobin) to 98.8% (azoxystrobin) for UV and 62.3% (trifloxystrobin) to 95.2% (azoxystrobin) for AD. In blanks spikes the recovery varied from 77.8% (picoxystrobin) to 88.4% (kresoxim-methyl) for UV and 76.7% (picoxystrobin) to 87.1% (dimoxystrobin) for AD. The method showed good precision (RSD < 10%). The results obtained by amperometric and UV detections were statistically comparable. Seven bean samples were analyzed to detect fungicide residues.

本文的目的是开发一种用硼掺杂的金刚石（BDD）电极与安培检测（AD）自制的高效液相色谱（HPLC / UV-Vis）结合的方法来测定六种链球菌素（杀菌剂）的方法。在C18反相色谱柱中分别使用200纳米和1.9伏的UV和AD检测器对杀菌剂进行HPLC分离。每个球蛋白的线性范围为5至15 mg L ^-1，所有化合物的相关系数均高于0.997。两种检测器均具有足够的可检测性（LOD为1.33至1.57μgkg ^-1）遵守监管机构预先设定的限制。经过验证，该方法具有良好的回收率和准确性。在加标样品中，UV的回收率范围为61.6％（三氟精氨酯）至98.8％（氮杂双氧乙烯），而AD的回收率介于62.3％（三氟精胺）至95.2％（三氟精胺）。在空白加标中，UV的回收率从77.8％（picoxystrobin）到88.4％（kresoxim-methyl），AD的回收率从76.7％（picoxystrobin）到87.1％（dimoxystrobin）。该方法显示出良好的精度（RSD <10％）。通过安培和紫外线检测获得的结果在统计学上可比。分析了七个豆样品以检测杀真菌剂残留。