Here, a novel iron-porphyrin-based covalent organic framework (COF_{p-Fepor NH2-BTA}) was synthesized and applied for electrochemical sensing H₂O₂ and pH which involved in many biological processes. The COF_{p-Fepor NH2-BTA} was obtained by post-modification of porphyrin-based COF (COF_{p-por NH2-BTA}) which was firstly synthesized by aldehyde-ammonia condensation reaction between 1,3,5-benzenetricarboxaldehyde and 5,10,15,20-tetrakis(4-aminophenyl)-21H,23H- porphine. The COF_{p-por NH2-BTA} was proved to be regular and uniform spherical particles with diameter about 1 μm, as well as possessed good crystalline structure and abundant micropores of about 1.4 nm. The resulted COF_{p-Fepor NH2-BTA} after post-modification with Fe²⁺ maintained the original shape and crystalline structure of COF_{p-por NH2-BTA}, while the micropores decreased to be about 0.89 nm. Electrochemical results indicated that the synthesized COF_{p-Fepor NH2-BTA} had good electrochemical redox and proton activity owing to iron-porphyrin, enabling to simultaneously be used as mimic peroxidase to catalyze the reduction of hydrogen peroxide (H₂O₂) and evaluate pH using current and potential as signal, respectively. The prepared sensor showed good performance for H₂O₂ detection from 6.85 nM to 7 μM with the detection limit of 2.06 nM (S/N = 3), and pH test from 3.0 to 9.0. This work demonstrated that the iron-porphyrin-based COF could be used as a mimic peroxidase to apply in biological fields.

在这里，合成了一种新型的基于铁卟啉的共价有机骨架（COF _{p -Fepor NH2-BTA}），并将其用于电化学传感H ₂ O ₂和pH值，这些过程涉及许多生物过程。的COF _{p -Fepor NH2-BTA}用的后修饰得到的卟啉系COF（COF _{p -POR NH2-BTA}），其首先由1,3,5- benzenetricarboxaldehyde和5,10-之间醛氨缩合反应合成，15,20-四（4-氨基苯基）-21H，23H-卟啉。COF_对-NH2-BTA被证明是规则且均匀的球形颗粒，直径约1μm，并具有良好的晶体结构和丰富的约1.4 nm的微孔。用Fe ²⁺进行后修饰后，_所得的COF _{p -Fepor NH2-BTA}保持了COF _{p -por NH2-BTA}的原始形状和晶体结构，而微孔减少至约0.89 nm。电化学结果表明，合成的COF _{p -Fepor NH2-BTA}由于具有铁卟啉，具有良好的电化学氧化还原和质子活性，可以同时用作模拟过氧化物酶催化过氧化氢（H ₂ O _2））并分别使用电流和电位作为信号评估pH。制备的传感器对H ₂ O _2的检测性能良好，检测限为6.85 nM至7μM，检测限为2.06 nM（S / N = 3），pH测试范围为3.0至9.0。这项工作表明，基于铁卟啉的COF可用作模拟过氧化物酶，可应用于生物领域。