Abstract The resolution of (R,S)-1-(4-methoxyphenyl) ethanol ((R,S)-MOPE) using an immobilized enzyme PM1000-TiO2-lipase in organic solvents was investigated. Under optimal conditions, the application of isopropyl as the organic solvent resulted in a reaction temperature of 40 °C, a ratio of (R,S)-MOPE to vinyl acetate of 2:1, the a water content of 0.56, a (R,S)-MOPE concentration of 200 mM, a (R,S)-MOPE conversion of 49.1 % and an enantiomer excess (ee) of (R)-ethyl acyl-MOPE of 99.1 %. These results indicate the high tolerance of immobilized lipase to the substrate with excellent enantioselectivity. The initial reaction rate increased with (R,S)-MOPE and vinyl acetate concentrations, thus a ping-pong Bi-Bi mechanism without inhibition model was selected for the (R,S)-MOPE resolution using PM1000-TiO2-lipase in the isopropyl ether. The fitting results of the kinetic parameters were as follows: a Michaelis constant for vinyl acetate KmA of 343.64 mM, a Michaelis constant for (R,S)-MOPE KmB of 104.81 mM, and a maximum reaction rate rmax of 0.49 mM min−1 mg−1. Moreover, the PM1000-TiO2-lipase demonstrated a high operational stability and maintained a relative activity of 86.0 % following 10 batch reactions.

中文翻译：

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使用具有高底物耐受性的固定化脂肪酶拆分外消旋 1-(4-甲氧基苯基) 乙醇

摘要 研究了在有机溶剂中使用固定化酶 PM1000-TiO2-脂肪酶对 (R,S)-1-(4-甲氧基苯基) 乙醇 ((R,S)-MOPE) 的拆分。在最佳条件下，以异丙基为有机溶剂，反应温度为 40 °C，(R,S)-MOPE 与醋酸乙烯酯的比例为 2:1，含水量为 0.56，a (R ,S)-MOPE 浓度为 200 mM，(R,S)-MOPE 转化率为 49.1%，(R)-乙基酰基-MOPE 的对映异构体过量 (ee) 为 99.1%。这些结果表明固定化脂肪酶对底物的高耐受性和优异的对映选择性。初始反应速率随着 (R,S)-MOPE 和醋酸乙烯酯浓度的增加而增加，因此选择了无抑制模型的乒乓 Bi-Bi 机制用于使用 PM1000-TiO2-脂肪酶的 (R,S)-MOPE 分辨率异丙醚。动力学参数的拟合结果如下：醋酸乙烯酯的米氏常数 KmA 为 343.64 mM，(R,S)-MOPE KmB 的米氏常数为 104.81 mM，最大反应速率 rmax 为 0.49 mM min-1毫克-1。此外，PM1000-TiO2-脂肪酶表现出很高的操作稳定性，并在 10 次分批反应后保持 86.0% 的相对活性。