An aroyl hydrazone based fluorescent probe, hpsh, has been developed for the selective detection of Cu²⁺ ions in pure aqueous medium by static fluorescence quenching. The fluorescence quenching of hpsh in the presence of Cu²⁺ takes place as a result of ground state complex formation through intramolecular charge transfer (ICT). Addition of Cu²⁺ ions changes the color of the solution from colorless to yellow-green which is clearly visible by naked eye. Large Stokes shift of hpsh prevents the self-quenching of the probe in absence of metal ions. The observed stoichiometry between Cu²⁺ and probe has been found as 1:2 (M: L). MTT assay of hpsh on fruit flies confirms that the probe is non-toxic and biocompatible. The plausible in vivo bioimaging application of the probe to detect Cu²⁺ in Drosophila gut tissues as well as in adult fruit fly has been investigated with excellent results.

已开发出一种基于芳酰的荧光探针hpsh，用于通过静态荧光猝灭选择性检测纯水介质中的Cu ²⁺离子。在Cu ²⁺存在下hpsh的荧光猝灭是通过分子内电荷转移（ICT）形成基态复合物的结果。Cu ²⁺离子的添加​​将溶液的颜色从无色变为黄绿色，用肉眼可以清楚看到。hpsh的较大斯托克斯位移可防止在没有金属离子的情况下探头自动熄灭。发现Cu ²⁺与探针之间的化学计量比为1：2（M：L）。实蝇的hpsh的MTT分析证实该探针无毒且具有生物相容性。合理的探针在体内生物成像中用于检测果蝇肠道组织以及成年果蝇中的Cu ²⁺的研究已经取得了优异的结果。