There have been reports of Helicobacter pylori (H. pylori) in the oral cavity and it has been suggested that the oral cavity may be a reservoir for H. pylori reflux from the stomach. High-throughput sequencing was used to assess the structure and composition of oral microbiota communities in individuals with or without confirmed H. pylori infection. Saliva samples were obtained from 34 H. pylori infected and 24 H. pylori uninfected subjects. Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatics analysis. Saliva sampling was repeated from 22 of the 34 H. pylori infected subjects 2 months after H. pylori eradication. High-quality sequences (2,812,659) clustered into 95,812 operational taxonomic units (OTUs; 97% identity). H. pylori was detected in the oral cavity in infected (12/34), uninfected (11/24) and eradicated (15/22) subjects by technique of high-throughput sequencing, occupying 0.0139% of the total sequences. Alpha diversity of H. pylori infected subjects was similar to that of uninfected subjects (Shannon: 1417.58 vs. 1393.60, p > 0.05, ACE: 1491.22 vs. 1465.97, p > 0.05, Chao 1: 1417.58 vs. 1393.60, p > 0.05, t-test). Eradication treatment decreased salivary bacterial diversity (Shannon, p = 0.015, ACE, p = 0.003, Chao 1, p = 0.002, t-test). Beta diversity analysis based on unweighted UniFrac distances showed that the salivary microbial community structure differed between H. pylori infected and uninfected subjects (PERMANOVAR, pseudo-F: 1.49, p = 0.033), as well as before and after H. pylori eradication (PERMANOVAR, pseudo-F: 3.34, p = 0.001). Using LEfSe analysis, 16 differentially abundant genera were defined between infected and uninfected subjects, 12 of which had a further alteration after successful eradication. Our study using high-throughput sequencing showed that H. pylori was present commonly in the oral cavity with no clear relation to H. pylori infection of the stomach. Both H. pylori infection and eradication therapy caused alterations in community and structure of the oral microbiota. clinicaltrials.gov, NCT03730766. Registered 2 Nov 2018 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/ NCT03730766.

中文翻译：

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通过高通量测序分析：幽门螺杆菌感染和唾液微生物组。

已有口腔中幽门螺杆菌（H.pylori）的报道，并且有人提出口腔可能是幽门螺杆菌从胃中回流的贮存器。高通量测序用于评估有或没有确诊幽门螺杆菌感染的个体口腔微生物群的结构和组成。从34名幽门螺杆菌感染者和24名未感染幽门螺杆菌的受试者获得唾液样品。提取细菌基因组DNA，并通过扩增16S rDNA V3-V4高变区进行测序，然后进行生物信息学分析。根除幽门螺杆菌2个月后，从34名受幽门螺杆菌感染的受试者中的22名重复进行唾液采样。高质量序列（2,812,659）聚集成95,812个操作生物分类单位（OTU； 97％相同）。H。通过高通量测序技术在感染（12/34），未感染（11/24）和根除（15/22）的受试者的口腔中检测到幽门螺杆菌，占总序列的0.0139％。幽门螺杆菌感染受试者的Alpha多样性与未感染受试者相似（Shannon：1417.58 vs. 1393.60，p> 0.05，ACE：1491.22 vs. 1465.97，p> 0.05，Chao 1：1417.58 vs. 1393.60，p> 0.05， t检验）。根除治疗降低了唾液细菌多样性（Shannon，p = 0.015，ACE，p = 0.003，Chao 1，p = 0.002，t检验）。基于未加权UniFrac距离的Beta多样性分析表明，幽门螺杆菌感染者和未感染者之间的唾液微生物群落结构不同（PERMANOVAR，伪F：1.49，p = 0.033），以及根除幽门螺杆菌前后（PERMANOVAR） ，假F：3.34，p = 0.001）。使用LEfSe分析，在感染和未感染的受试者之间定义了16个差异丰富的属，其中12个在成功根除后有进一步的改变。我们使用高通量测序的研究表明，幽门螺杆菌通常存在于口腔中，与胃中的幽门螺杆菌感染没有明显关系。幽门螺杆菌感染和根除疗法均引起口腔微生物群和结构的改变。Clinicaltrials.gov，NCT03730766。注册于2018年11月2日-追溯注册，https：//clinicaltrials.gov/ct2/show/ NCT03730766。幽门螺杆菌常见于口腔中，与胃中的幽门螺杆菌感染没有明显关系。幽门螺杆菌感染和根除疗法均引起口腔微生物群和结构的改变。Clinicaltrials.gov，NCT03730766。注册于2018年11月2日-追溯注册，https：//clinicaltrials.gov/ct2/show/ NCT03730766。幽门螺杆菌常见于口腔中，与胃中的幽门螺杆菌感染没有明显关系。幽门螺杆菌感染和根除疗法均引起口腔微生物群和结构的改变。Clinicaltrials.gov，NCT03730766。注册于2018年11月2日-追溯注册，https：//clinicaltrials.gov/ct2/show/ NCT03730766。