Perfluorooctanesulfonate (PFOS) is a common environmental pollutant and has been detected in various environmental media. Several major isomers have been identified, and their profiles provide important signatures from the source of contamination. Gas chromatography has a significant resolving power for the isomers. However, methods for derivatizing sulfonates for a sensitive analysis are limited. In this study, in-port derivatization with diaryl iodonium salts into aryl sulfonates was used. A technical mixture of PFOS was separated on gas chromatography to afford 11 isomers. In electron-capture negative ionization, the PFOS derivatives form specific fragmented anions (sulfinate anions). The derivatization was also applied to other per- or polyfluoroalkyl acids (PFASs; 13 perfluoroalkyl carboxylic acids and 10 sulfonates). Analysis of human serum samples was achieved with as little as 50 μL of sample and indicated the presence of PFOS isomers and PFASs. The detection limits of PFOS isomers were 0.02–0.09 ng mL^–1 with sufficient recoveries (85%–98%), and those for other PFASs ranged from 0.01–1 ng mL^–1 (recoveries, 80%–100%), which are suitable for the analysis of human serum samples.

中文翻译：

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入口充气化气相色谱-负化学电离-质谱法分析血清中的全氟辛烷磺酸异构体和其他全氟烷基酸

全氟辛烷磺酸（PFOS）是一种常见的环境污染物，已在各种环境介质中检测到。已经鉴定出几种主要的异构体，它们的谱提供了来自污染源的重要特征。气相色谱对异构体具有显着的分辨能力。但是，用于灵敏分析的磺酸盐衍生化方法受到限制。在这项研究中，使用了二芳基碘鎓盐进行港口衍生化为芳基磺酸盐。在气相色谱上分离出全氟辛烷磺酸的工业混合物，得到11种异构体。在电子捕获负电离中，PFOS衍生物形成特定的碎片阴离子（亚磺酸根阴离子）。衍生化还应用于其他全氟或多氟烷基酸（PFAS； 13种全氟烷基羧酸和10种磺酸盐）。只需低至50μL的样品即可完成人血清样品的分析，表明存在PFOS异构体和PFAS。PFOS异构体的检出限为0.02–0.09 ng mL^–1具有足够的回收率（85％–98％），其他PFAS的回收率范围为0.01–1 ng mL ^–1（回收率80％–100％），适用于分析人类血清样品。