A 60‐day experiment was carried out to investigate dietary starch levels on growth performance, hepatic glucose metabolism and liver histology of largemouth bass, Micropterus salmoides. Fish (initial weight 22.00 ± 0.02 g) were fed five graded levels of dietary corn starch (0, 50, 100, 150 and 200 g/kg). Fish fed low (0 and 50 g/kg) dietary starch showed significantly higher weight gain than other groups (p < .05). Liver lipid and glycogen accumulations were induced when dietary starch higher than 100 g/kg. After 20 days of feeding, hexokinase activity and mRNA expression were decreased in fish fed dietary starch higher than 150 g/kg (p < .05) and the pyruvate kinase showed the opposite tendency. Insulin receptor 1 (irs1), glucagon‐like peptide‐1 receptor and glucose transport protein 2 (glut2) mRNA expression were decreased with the increasing dietary starch after 10 days of feeding (p < .05). These results indicated gluconeogenesis was depressed and β‐oxidation was enhanced in response to high dietary starch, while the glycolysis was inhibited and endocrine system was impaired when fish fed high dietary starch; then, glucose homeostasis was disturbed and finally led to the glucose intolerance of largemouth bass.

中文翻译：

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高膳食淀粉会损害幼年大嘴鲈，沙门氏菌的生长性能，肝脏组织学和肝葡萄糖代谢

进行了为期60天的实验，以研究膳食淀粉水平对大嘴鲈，小鳍鲈的生长性能，肝葡萄糖代谢和肝脏组织学的影响。向鱼（初始重量22.00±0.02克）喂食五级玉米淀粉（0、50、100、150和200 g / kg）。饲喂低（0和50 g / kg）饮食淀粉的鱼的增重明显高于其他组（p  <.05）。当膳食淀粉高于100 g / kg时，会引起肝脏脂质和糖原积累。饲喂20天后，饲喂日粮淀粉含量超过150 g / kg的鱼的己糖激酶活性和mRNA表达下降（p  <.05），丙酮酸激酶显示出相反的趋势。胰岛素受体1（irs1），饲喂10天后，随着日粮淀粉含量的增加，胰高血糖素样肽-1受体和葡萄糖转运蛋白2（glut2）mRNA的表达降低（p  <.05）。这些结果表明，饲喂高膳食淀粉的鱼，糖异生作用降低，β-氧化作用增强，而饲喂高膳食淀粉的鱼的糖酵解作用受到抑制，内分泌系统受损。然后，葡萄糖体内稳态受到干扰，最终导致大嘴鲈鱼对葡萄糖的不耐受。