This study examined the effects of black cumin seed (BCS) on growth performance, whole‐body proximate and fatty acid composition, and blood biochemistry of Pacific white shrimp Litopenaeus vannamei. Six hundred shrimp (7.5 ± 0.2 g) were assigned into five treatments in three replicates. Five diets containing BCS at 0, 5, 15, 30 and 50 g/kg were prepared and named BCS0, BCS5, BCS15, BCS30 and BCS50, respectively. Shrimp were fed at a rate of 5% body weight three times daily for 90 days. Shrimp fed BCS5, BCS15 and BCS30 showed no adverse effects on growth, whereas shrimp fed BCS50 showed retarded growth parameters. Consistently, cortisol was significantly increased in BCS50 group. Moreover, cholesterol and triglyceride were significantly lower in shrimp that fed diets containing BCS compared to the control (p < .05). The highly unsaturated fatty acid (HUFA) was significantly higher in the control diet compared to other diets, but it was significantly higher in shrimp that fed BCS30 and BCS50 than in control shrimp with no significant increase in Δ6 fatty acyl desaturase transcript. Considering the growth parameters, the highest body HUFA contents, and the lowest haemolymph cholesterol and triglyceride levels, the supplementation of BCS in shrimp diet is suggested at 30 g/kg.

中文翻译：

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日粮黑孜然粉对南美白对虾凡纳滨对虾生长性能，血液生化和脂肪酸组成的影响

这项研究探讨了黑孜然种子（BCS）对太平洋白虾凡纳滨对虾的生长性能，全身邻近和脂肪酸组成以及血液生化的影响。将六百只虾（7.5±0.2克）分成三份重复进行五次处理。制备了五种含BCS的饮食，分别为0、5、15、30和50 g / kg，分别命名为BCS0，BCS5，BCS15，BCS30和BCS50。虾以5％体重的速率喂食90天，每天3次。虾饲喂BCS5，BCS15和BCS30对生长无不利影响，而虾饲喂BCS50显示生长参数受阻。始终如一，BCS50组皮质醇显着增加。此外，与对照相比，饲喂含BCS的虾对虾中的胆固醇和甘油三酯显着降低（p  <.05）。对照日粮中的高度不饱和脂肪酸（HUFA）与其他日粮相比明显更高，但饲喂BCS30和BCS50的虾中的非饱和脂肪酸明显高于对照虾，而Δ6脂肪酰基去饱和酶转录物没有明显增加。考虑到生长参数，最高的人体HUFA含量以及最低的血淋巴胆固醇和甘油三酯水平，建议在虾饲料中添加BCS 30 g / kg。