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Karanj protein isolate prepared from karanj seed cake: Effect on growth, body composition and physiometabolic responses in Labeo rohita fingerlings
Aquaculture Nutrition ( IF 3.5 ) Pub Date : 2019-12-22 , DOI: 10.1111/anu.13033
Amrutha Gopan 1 , Narottam P. Sahu 1 , Tincy Varghese 1 , Parimal Sardar 1 , Manas K. Maiti 1
Affiliation  

A protein isolate was prepared from karanj seed (KPI) with 921.2 g protein/kg seed, which contained a negligible amount of anti‐nutritional factors and a balanced amino acid composition, especially rich in methionine. For 60‐day feeding trial, five isonitrogenous (300 g/kg CP) and isocaloric (15 MJ DE/kg) diets were formulated by replacing soybean protein isolate (SPI) on protein equivalent basis, KPI‐0 (control, 0 g/kg KPI); KPI‐25 (replacing 250 g/kg SPI protein with KPI); KPI‐50 (replacing 500 g/kg SPI protein with KPI); KPI‐75 (replacing 750 g/kg SPI protein with KPI) and KPI‐100 (replacing 1,000 g/kg SPI protein with KPI) for the feeding of L. rohita. The weight gain percentage, specific growth rate, feed conversion ratio and protein efficiency ratio were not significantly (p > .05) varied among the KPI fed and control groups. A significantly higher hepatosomatic index was recorded in the control and KPI‐25 groups compared with other groups. The whole‐body compositions, except ether extract, did not differ significantly (p > .05) among the groups. Digestive (amylase, protease, lipase and alkaline phosphatase) and metabolic enzyme activities (hexokinase, transaminases and lactate dehydrogenase) and glycogen stores were not significantly affected, whereas intestinal alkaline phosphatase differed significantly (p < .05). The RNA–DNA ratio was significantly (p < .05) higher in the KPI‐75 group. Thus, the study revealed that KPI can completely replace SPI protein at 191 g/kg inclusion level in the diets of L. rohita fingerlings.

中文翻译:

从 karanj 种子饼制备的 Karanj 蛋白分离物:对 Labeo rohita 鱼种生长、身体成分和生理代谢反应的影响

从 karanj 种子 (KPI) 中制备了蛋白质分离物,蛋白质含量为 921.2 g/kg 种子,其中含有可忽略不计的抗营养因子和平衡的氨基酸组成,特别是富含蛋氨酸。对于 60 天的饲喂试验,在蛋白质当量的基础上,KPI-0(对照,0 g/kg)替代大豆分离蛋白(SPI),配制了五种等氮(300 g/kg CP)和等热量(15 MJ DE/kg)日粮。公斤 KPI);KPI-25(用KPI代替250 g/kg SPI蛋白);KPI-50(用KPI代替500 g/kg SPI蛋白);KPI-75(用 KPI 代替 750 g/kg SPI 蛋白)和 KPI-100(用 KPI 代替 1,000 g/kg SPI 蛋白)用于喂养 L. rohita。KPI 喂养组和对照组之间的增重百分比、比生长率、饲料转化率和蛋白质效率比没有显着差异 (p > .05)。与其他组相比,对照组和 KPI-25 组的肝体指数显着更高。除乙醚提取物外,各组的全身成分没有显着差异(p > .05)。消化(淀粉酶、蛋白酶、脂肪酶和碱性磷酸酶)和代谢酶活性(己糖激酶、转氨酶和乳酸脱氢酶)和糖原储存没有受到显着影响,而肠道碱性磷酸酶有显着差异(p < .05)。KPI-75 组的 RNA-DNA 比率显着(p < .05)更高。因此,该研究表明,KPI 可以在 L. rohita 鱼种饲料中以 191 g/kg 的含量完全替代 SPI 蛋白。除乙醚提取物外,各组之间没有显着差异(p > .05)。消化(淀粉酶、蛋白酶、脂肪酶和碱性磷酸酶)和代谢酶活性(己糖激酶、转氨酶和乳酸脱氢酶)和糖原储存没有受到显着影响,而肠道碱性磷酸酶有显着差异(p < .05)。KPI-75 组的 RNA-DNA 比率显着(p < .05)更高。因此,该研究表明,KPI 可以在 L. rohita 鱼种饲料中以 191 g/kg 的含量完全替代 SPI 蛋白。除乙醚提取物外,各组之间没有显着差异(p > .05)。消化(淀粉酶、蛋白酶、脂肪酶和碱性磷酸酶)和代谢酶活性(己糖激酶、转氨酶和乳酸脱氢酶)和糖原储存没有受到显着影响,而肠道碱性磷酸酶有显着差异(p < .05)。KPI-75 组的 RNA-DNA 比率显着(p < .05)更高。因此,该研究表明,KPI 可以在 L. rohita 鱼种饲料中以 191 g/kg 的含量完全替代 SPI 蛋白。而肠道碱性磷酸酶差异显着(p < .05)。KPI-75 组的 RNA-DNA 比率显着(p < .05)更高。因此,该研究表明,KPI 可以在 L. rohita 鱼种饲料中以 191 g/kg 的含量完全替代 SPI 蛋白。而肠道碱性磷酸酶差异显着(p < .05)。KPI-75 组的 RNA-DNA 比率显着(p < .05)更高。因此,该研究表明,KPI 可以在 L. rohita 鱼种饲料中以 191 g/kg 的含量完全替代 SPI 蛋白。
更新日期:2019-12-22
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