A 65‐day feeding trial was conducted to investigate the effects of dietary antimicrobial peptide APSH‐07 on the growth performance, anti‐oxidation responses, stress resistance and intestine microbiota of large yellow croaker Larimichthys crocea. Four isonitrogenous and isolipidic diets were formulated with 0, 30, 60 and 90 mg/kg of APSH‐07, respectively. The results showed that the fish fed with 90 mg/kg of dietary APSH‐07 had the significantly highest specific growth rate (p < .05). The activities of catalase, superoxide dismutase, the total anti‐oxidative capacity and lysozyme had the significantly highest values in liver of fish fed with 90 mg/kg of dietary APSH‐07 (p < .05). Serum glucose increased significantly in fish fed diets without APSH‐07 supplementation after temperature stress, while serum cortisol increased significantly in those after trawl stress (p < .05). Fish fed with 90 mg/kg of dietary APSH‐07 showed significantly higher operational taxonomic units, ACE estimator and phylogenetic diversity whole tree in intestine microbiota compared to fish fed without APSH‐07 (p < .05). In conclusion, under the present experimental condition, 90 mg/kg of antimicrobial peptide APSH‐07 supplementation in diet had the better growth performance, higher anti‐oxidation and stress resistance capacity, and a potentially more beneficial intestine microbial community of large yellow croaker.

中文翻译：

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抗菌肽APSH-07对大黄鱼生长性能、抗氧化反应、抗逆性和肠道菌群的影响

一项为期 65 天的饲养试验旨在研究膳食抗菌肽 APSH-07 对大黄鱼 Larimichthys crocea 生长性能、抗氧化反应、抗逆性和肠道微生物群的影响。四种异氮和异脂饮食分别用 0、30、60 和 90 mg/kg 的 APSH-07 配制。结果表明，饲喂 90 mg/kg 膳食 APSH-07 的鱼具有最高的比生长率（p < .05）。过氧化氢酶、超氧化物歧化酶、总抗氧化能力和溶菌酶的活性在饲喂 90 mg/kg 饲料 APSH-07 的鱼的肝脏中显着最高（p < .05）。在温度应激后，未添加 APSH-07 的鱼饲料中血清葡萄糖显着增加，而拖网应激后血清皮质醇显着增加（p < .05）。与未饲喂 APSH-07 的鱼相比，饲喂 90 mg/kg 膳食 APSH-07 的鱼在肠道微生物群中显示出显着更高的操作分类单位、ACE 估计量和系统发育多样性整棵树（p < .05）。综上所述，在目前的实验条件下，日粮中添加 90 mg/kg 抗菌肽 APSH-07 具有更好的生长性能、更高的抗氧化和抗逆能力，以及潜在更有益的大黄鱼肠道微生物群落。与未饲喂 APSH-07 的鱼相比，肠道微生物群中整棵树的 ACE 估计量和系统发育多样性（p < .05）。综上所述，在目前的实验条件下，日粮中添加 90 mg/kg 抗菌肽 APSH-07 具有更好的生长性能、更高的抗氧化和抗逆能力，以及潜在更有益的大黄鱼肠道微生物群落。与未饲喂 APSH-07 的鱼相比，肠道微生物群中整棵树的 ACE 估计量和系统发育多样性（p < .05）。综上所述，在目前的实验条件下，日粮中添加 90 mg/kg 抗菌肽 APSH-07 具有更好的生长性能、更高的抗氧化和抗逆能力，以及潜在更有益的大黄鱼肠道微生物群落。