Two feeding trials were conducted to investigate the effect of replacing fishmeal with a combination of soy and corn protein concentrate (1:1 ratio) on growth performance of the Pacific white shrimp (Litopenaeus vannamei). A basal diet containing 200 g/kg fishmeal was systematically reduced (200, 150, 100, 50 and 0 g/kg) with protein concentrate on an isonitrogenous basis. Additionally, two diets containing 0 or 50 g/kg fishmeal were supplemented with lysine and methionine to evaluate possible limitations in EAAs. Each diet was randomly fed to five replicate tanks (15 shrimp per 75 L aquaria) reared in an indoor clear water system (Trial 1), or four replicate circular tanks (100 shrimp per 800 L) reared in outdoor green water system (Trial 2). In trial 1, results indicated a slight decrease in shrimp performance as fishmeal was replaced at the highest levels. Meanwhile, the supplementation of lysine and methionine to the diets did not result in shifts in survival, growth or FCR. In trial 2, there were no significant differences in growth performance across the tested diets. This study demonstrated that plant‐based protein concentrates can be used to replace fishmeal in practical shrimp diet in clear and green water under high stocking density.

中文翻译：

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在高放养密度和低盐度条件下养殖的南美白对虾（Litopenaeus vannamei）的实际日粮中使用植物蛋白浓缩物替代鱼粉

进行了两项饲养试验，以研究用大豆和玉米浓缩蛋白的组合（比例为 1:1）代替鱼粉对南美白对虾（Litopenaeus vannamei）生长性能的影响。含有 200 g/kg 鱼粉的基础日粮在等氮基础上通过蛋白质浓缩物系统地减少（200、150、100、50 和 0 g/kg）。此外，两种含有 0 或 50 g/kg 鱼粉的日粮添加了赖氨酸和甲硫氨酸，以评估 EAA 的可能限制。将每种日粮随机喂入在室内清水系统（试验 1）中饲养的 5 个重复池（每 75 L 水族箱 15 只虾），或在室外绿水系统（试验 2）中饲养的四个重复圆形池（每 800 L 饲养 100 只虾） ）。在试验 1 中，结果表明，随着鱼粉在最高水平上被替代，虾的生产性能略有下降。同时，在日粮中补充赖氨酸和蛋氨酸不会导致存活率、生长率或 FCR 的变化。在试验 2 中，测试日粮的生长性能没有显着差异。该研究表明，在高放养密度的清澈绿水中，植物蛋白浓缩物可用于替代实际虾饲料中的鱼粉。