Dietary copper requirement of Heteropneustes fossilis (6.74 ± 0.03 g) was determined by feeding purified diets containing same protein (400 g/kg) and gross energy (17.89 kJ/g) but different levels of copper for 12 weeks. Graded amount of CuSO₄.5H₂O (0, 1.96, 3.93, 5.89, 7.86, 9.82, 11.79 mg/kg) was supplemented to basal diet to attain desired dietary copper levels (0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/kg). Analysed dietary copper concentrations were 4.28, 4.63, 5.28, 5.70, 6.19 and 6.69 mg/kg. Absolute weight gain, feed conversion ratio and protein gain improved with the increasing levels of dietary copper up to 5.28 mg/kg. Further inclusion of copper at a level of 5.70 mg/kg did not improve the above parameters. Significantly higher (p < .05) plasma ceruloplasmin, liver copper‐zinc superoxide dismutase, catalase activities and lower thiobarbituric acid reactive substances were evident in fish receiving diets with 5.28 and 5.70 mg/kg copper compared to other groups. Whole body and liver copper concentrations increased significantly (p < .05) with increasing dietary copper levels. Quadratic regression analysis of absolute weight gain, feed conversion ratio, protein gain and broken‐line regression analysis of plasma ceruloplasmin activity and liver TBARS value against the variable dietary copper levels depicted the dietary copper requirements for fingerling H. fossilis in the range of 5.24–5.68 mg/kg.

中文翻译：

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用于配制铜平衡商业饲料的化石异龙鱼鱼种的膳食铜需求

通过饲喂含有相同蛋白质 (400 g/kg) 和总能量 (17.89 kJ/g) 但铜含量不同的纯化日粮 12 周来确定 Heteropneustes stoneis (6.74 ± 0.03 g) 的日粮铜需求量。将分级量的 CuSO4.5H2O（0、1.96、3.93、5.89、7.86、9.82、11.79 mg/kg）添加到基础日粮中，以达到所需的日粮铜水平（0、0.5、1.0、1.5、2.0 和 .30.2.0 毫克）。 /公斤）。分析的膳食铜浓度为 4.28、4.63、5.28、5.70、6.19 和 6.69 mg/kg。随着日粮铜含量增加至 5.28 mg/kg，绝对增重、饲料转化率和蛋白质增加得到改善。进一步包含 5.70 mg/kg 水平的铜并没有改善上述参数。血浆铜蓝蛋白、肝铜锌超氧化物歧化酶显着更高 (p < .05)，与其他组相比，在接受 5.28 和 5.70 mg/kg 铜饮食的鱼中，过氧化氢酶活性和硫代巴比妥酸活性物质较低。随着膳食铜含量的增加，全身和肝脏铜浓度显着增加（p < .05）。绝对增重、饲料转化率、蛋白质增重的二次回归分析以及血浆铜蓝蛋白活性和肝脏 TBARS 值相对于可变日粮铜水平的折线回归分析描述了鱼种化石 H.化石的日粮铜需求量在 5.24– 范围内5.68 毫克/公斤。