Japanese flounder juveniles (initial body weight: 7.12 ± 0.02 g) were fed three diets containing 0, 120 and 200 g/kg of carbohydrates (C0, C120 and C200, respectively) for 10 weeks. Results showed that higher dietary carbohydrate intake enabled further deposition of glycogen and lipids in liver and muscle. The mRNA levels of glucokinase (gck), phosphofructokinase (pfkl) and hexokinase (HK) activity involved in glycolysis were significantly up‐regulated in C120 (p .05) but significantly increased in C200 group (p < .05). In conclusion, Japanese flounder was able to use carbohydrates efficiently through regulation of glucose and lipid metabolism when dietary carbohydrate was not higher than 120 g/kg, while 200 g/kg of dietary carbohydrate caused the deregulation of glucose homoeostasis.

中文翻译：

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幼年日本比目鱼 Paralichthys olivaceus 糖脂代谢对不同膳食碳水化合物水平的分子适应性

日本比目鱼幼鱼（初始体重：7.12 ± 0.02 g）被喂食三种含有 0、120 和 200 g/kg 碳水化合物（分别为 C0、C120 和 C200）的饮食，持续 10 周。结果表明，较高的膳食碳水化合物摄入量能够进一步沉积肝和肌肉中的糖原和脂质。参与糖酵解的葡萄糖激酶 (gck)、磷酸果糖激酶 (pfkl) 和己糖激酶 (HK) 的 mRNA 水平在 C120 中显着上调 (p = .05)，但在 C200 组中显着增加 (p < .05)。综上所述，当日粮碳水化合物不高于 120 g/kg 时，日本比目鱼能够通过调节糖脂代谢有效利用碳水化合物，而 200 g/kg 日粮碳水化合物会导致葡萄糖稳态失调。