BACKGROUND The aim of the current resarch to clarify ensiling of whole plant Hybrid pennisetum with Natamycin and Lactobacillus plantarum impacts on fermentation characteristics and meta-genomic microbial community at low temperature. RESULTS During ensiling process, lactic acid (LA) and lactic acid bacteria (LAB) was significantly (P < 0.05) increased and acetic acid (AA), water-soluble carbohydrate (WSC), ammonia total nitrogen (NH3-N) and yeast significantly (P < 0.05) reduced in treatments as compared to control. The bacterial community with different treatment and ensiling days variations showed on family and genus levels. The family Lactobacillaceae and genus Lactobacillus and Pediococcus bacterial community have dominant in inculcated silage. The bacterial ecology and fermentation quality with additives and ensiling day can be confirmed by principal component analysis (PCA) at the family and genus levels. The PCO1, and PCO2 can be explained by 10.81 % and 72.14 % of the whole variance regularly, similarly in PCO1 and PCO2 can be explained 24.23 % and 52.06 % regularly. The bacterial core micro-biome OTUs numbers were increased in treatments as compared to control at different ensiling days of Hybrid pennisetum silage. CONCLUSIONS It is detected that the inoculation of L. plantarum alone and combined with natamycin influenced the fermentation quality and reduced undesirable microorganisms during the fermentation process of Hybrid pennisetum silage. Natamycin alone did not enhance the organic acid concentration but numerically enhanced as compared to control. This article is protected by copyright. All rights reserved.

中文翻译：

---

纳他霉素和植物乳杆菌全株杂交狼尾草青贮对低温发酵特性和宏基因组微生物群落的影响

背景目前研究的目的是阐明具有纳他霉素和植物乳杆菌的全植物杂交狼尾草青贮对低温下发酵特性和宏基因组微生物群落的影响。结果青贮过程中乳酸（LA）和乳酸菌（LAB）显着升高（P < 0.05），乙酸（AA）、水溶性碳水化合物（WSC）、氨总氮（NH3-N）和酵母菌与对照相比，处理显着降低（P < 0.05）。不同处理和青贮天数变化的细菌群落表现在科和属水平上。乳杆菌科、乳杆菌属和片球菌属细菌群落在青贮青贮饲料中占优势。加入添加剂和青贮日的细菌生态学和发酵质量可以通过科和属水平的主成分分析（PCA）来确认。PCO1 和 PCO2 可以有规律地解释为整个方差的 10.81 % 和 72.14 %，同样在 PCO1 和 PCO2 中可以有规律地解释 24.23 % 和 52.06 %。在杂交狼尾草青贮的不同青贮天数下，与对照相比，处理中细菌核心微生物组 OTU 数量增加。结论经检测，在杂交狼尾草青贮发酵过程中，单独接种植物乳杆菌和与游霉素联合接种可影响发酵质量，减少不良微生物。与对照相比，单独的游霉素没有提高有机酸浓度，而是在数值上提高了。本文受版权保护。版权所有。