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Grh signaling regulates epithelium development and ecdysis in Blattella germanica
Insect Science ( IF 4 ) Pub Date : 2020-07-20 , DOI: 10.1111/1744-7917.12780
Zheng Zhao 1, 2 , Liang Li 1, 2 , Min Cheng 1, 2 , An-Di Jing 1, 2 , Su-Ning Liu 1, 2 , Shi-Ming Zhu 1, 2 , Er-Xia Du 1, 2 , Sheng Li 1, 2 , Yun-Xia Luan 1, 2 , Chong-Hua Ren 1, 2
Affiliation  

The transcription factor grainy head (Grh) functions in the protection of the epithelium against the external environment by generating strongly adhesive layers, and this function is conserved in vertebrates and invertebrates. In Drosophila, the top model for holometabolous insects, Grh is necessary during embryonic development, epidermal differentiation, central nervous system specification and epithelial repair. However, the function of this gene in hemimetabolous insect epithelia remains unknown. To examine the function of Grh signaling in regulating epithelium development in Hemimetabola, we focused on the Blattella germanica epidermal layer using a gene knockdown strategy. The spatiotemporal expression pattern of BgGrh was detected, and knockdown of BgGrh and BgCad96ca, which provide positive feedback to BgGrh, caused severe defects in new epithelium development and impeded the molting process required to discard the old integument. Knockdown of the expression of BgGrh and BgCad96ca caused increased expression of chitin synthase gene (BgCHS1) and chitinase gene (BgCht5), whose upregulations should be mediated by the higher level of hormone receptor 3 (BgHr3) gene. In conclusion, epithelium development is regulated by Grh signaling, which might represent a potential target for the control of the urban pest cockroaches. This article is protected by copyright. All rights reserved.

中文翻译:

Grh 信号调节德国小蠊上皮发育和蜕皮

转录因子粒状头 (Grh) 通过产生强粘附层来保护上皮免受外部环境的影响,并且该功能在脊椎动物和无脊椎动物中是保守的。在果蝇中,全代谢昆虫的顶级模型,Grh 在胚胎发育、表皮分化、中枢神经系统规范和上皮修复过程中是必需的。然而,该基因在半代谢昆虫上皮细胞中的功能仍然未知。为了检查 Grh 信号在调节 Hemimetabola 上皮发育中的功能,我们使用基因敲低策略专注于德国小蠊表皮层。检测到 BgGrh 的时空表达模式,BgGrh 和 BgCad96ca 的敲低,为 BgGrh 提供正反馈,导致新上皮发育的严重缺陷,并阻碍了丢弃旧外皮所需的蜕皮过程。BgGrh 和 BgCad96ca 表达的抑制导致几丁质合酶基因 (BgCHS1) 和几丁质酶基因 (BgCht5) 的表达增加,其上调应由较高水平的激素受体 3 (BgHr3) 基因介导。总之,上皮发育受 Grh 信号的调节,这可能是控制城市害虫蟑螂的潜在目标。本文受版权保护。版权所有。其上调应由较高水平的激素受体 3 (BgHr3) 基因介导。总之,上皮发育受 Grh 信号的调节,这可能是控制城市害虫蟑螂的潜在目标。本文受版权保护。版权所有。其上调应由较高水平的激素受体 3 (BgHr3) 基因介导。总之,上皮发育受 Grh 信号的调节,这可能是控制城市害虫蟑螂的潜在目标。本文受版权保护。版权所有。
更新日期:2020-07-20
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