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A novel crosslinker-free technique toward the fabrication of collagen microspheres.
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.4 ) Pub Date : 2020-03-19 , DOI: 10.1002/jbm.b.34608
Colten Snider 1 , Mitch Bellrichard 2 , Amber Meyer 3 , Raghuraman Kannan 1, 4 , Dave Grant 1 , Sheila Grant 1
Affiliation  

Injectable collagen microspheres (CMs) have the potential to be an excellent tool to deliver various modulatory agents or to be used as a cellular transporter. A drawback has been the difficulty in producing reliable and spherical CMs. A crosslinker‐free method to fabricate CMs was developed using liquid collagen (LC) in a water‐in‐oil emulsion process with varying concentrations of surfactant span‐80. Different emulsion times of up to 16‐hr were utilized to produce the CMs. Visual microscopy and scanning electron microscopy were utilized to determine the morphology of the CMs. To determine the fibril nature of the CMs, focus ion beam milling, energy dispersive spectroscopy, and Fourier Transformation‐Infrared spectroscopy were performed. A cell biocompatibility study was performed to assess the biocompatibility of the CMs. The results demonstrated that consistent spherical CMs were achievable by changing the span‐80 concentration. The CMs were fibrilized not only at the surface, but also at the core. Both the 1‐ and 16‐hr emulsion time demonstrated biocompatibility and it appeared that the cells preferentially adhered to the CMs. This crosslinker‐free method to fabricate CMs resulted in spherical, stable, biocompatible CMs, and could be an excellent technique for multiple tissue engineering applications.

中文翻译:

一种用于制造胶原微球的新型无交联剂技术。

可注射的胶原微球 (CM) 有可能成为提供各种调节剂或用作细胞转运蛋白的绝佳工具。一个缺点是难以生产可靠的球形 CM。在油包水乳液工艺中使用液体胶原蛋白 (LC) 开发了一种不含交联剂的制备 CMs 的方法,具有不同浓度的表面活性剂 span-80。使用长达 16 小时的不同乳化时间来生产 CM。使用目视显微镜和扫描电子显微镜来确定 CM 的形态。为了确定 CM 的原纤维性质,进行了聚焦离子束研磨、能量色散光谱和傅里叶变换红外光谱。进行细胞生物相容性研究以评估 CM 的生物相容性。结果表明,通过改变 span-80 浓度可以获得一致的球形 CM。CM 不仅在表面,而且在核心处被原纤化。1 小时和 16 小时的乳化时间都证明了生物相容性,而且细胞似乎优先粘附在 CM 上。这种制造 CMs 的无交联剂方法产生了球形、稳定、生物相容性的 CMs,并且可能是多种组织工程应用的优秀技术。
更新日期:2020-03-19
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