Dynamic‐based systems are bio‐designed in order to mimic the micro‐environments of the bone tissue. There is limited direct comparison between perfusion and perfusion‐rotation forces in designing a bioreactor. Hence, in current study, we aimed to compare given bioreactors for bone regeneration. Two types of bioreactors including rotating & perfusion and perfusion bioreactors were designed. Mesenchymal stem cells derived from buccal fat pad were loaded on a gelatin/β‐Tricalcium phosphate scaffold. Cell‐scaffold constructs were subjected to different treatment condition and place in either of the bioreactors. Effect of different dynamic conditions on cellular behavior including cell proliferation, cell adhesion, and osteogenic differentiation were assessed. Osteogenic assessment of scaffolds after 24 days revealed that rotating & perfusion bioreactor led to significantly higher expression of OCN and RUNX2 genes and also greater amount of ALP and collagen I protein production compared to static groups and perfusion bioreactor. Observation of cellular sheets which filled the scaffold porosities in SEM images, approved the better cell responses to rotating & perfusion forces of the bioreactor. The outcomes demonstrated that rotating & perfusion bioreactor action on bone regeneration is much preferable than perfusion bioreactor. Therefore, it seems that exertion of multi‐stimuli is more effective for bone engineering.

中文翻译：

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工程骨组织的生物反应器培养条件：各种生物反应器设计对细胞外基质合成的影响。

基于动态的系统是生物设计的，以模拟骨组织的微环境。在设计生物反应器时，灌注和灌注-旋转力之间的直接比较有限。因此，在目前的研究中，我们旨在比较给定的生物反应器进行骨再生。设计了旋转灌注式生物反应器和灌注式生物反应器两种类​​型的生物反应器。取自颊脂肪垫的间充质干细胞装载在明胶/β-磷酸三钙支架上。细胞支架结构经受不同的处理条件并放置在任一生物反应器中。评估了不同动态条件对细胞行为的影响，包括细胞增殖、细胞粘附和成骨分化。24 天后支架的成骨评估显示旋转和 与静态组和灌注生物反应器相比，灌注生物反应器导致 OCN 和 RUNX2 基因显着更高的表达以及更多的 ALP 和胶原蛋白 I 蛋白的产生。在 SEM 图像中观察填充支架孔隙的细胞片，证实细胞对生物反应器的旋转和灌注力有更好的反应。结果表明，旋转和灌注生物反应器对骨再生的作用比灌注生物反应器更可取。因此，似乎多刺激的施加对骨工程更有效。在 SEM 图像中观察填充支架孔隙的细胞片，证实细胞对生物反应器的旋转和灌注力有更好的反应。结果表明，旋转和灌注生物反应器对骨再生的作用比灌注生物反应器更可取。因此，似乎多刺激的施加对骨工程更有效。在 SEM 图像中观察填充支架孔隙的细胞片，证实细胞对生物反应器的旋转和灌注力有更好的反应。结果表明，旋转和灌注生物反应器对骨再生的作用比灌注生物反应器更可取。因此，似乎多刺激的施加对骨工程更有效。