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Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay
Food and Agricultural Immunology ( IF 3 ) Pub Date : 2020-01-01 , DOI: 10.1080/09540105.2019.1708272
Jianqiang Lan 1, 2 , Weiming Sun 1, 2 , Lijun Chen 3 , Hailong Zhou 2 , Yongmei Fan 4 , Xiaoping Diao 2 , Baomin Wang 5 , Hongwei Zhao 1, 2, 4
Affiliation  

ABSTRACT It is long-cherished to develop rapid approaches for the identification and quantification of pesticide and its metabolites. Carbofuran (CBF) and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are highly toxic to humans and non-target organisms, which makes it mandatory for the detection of their residues in foods and the environment in many countries. Herein, a colloidal gold-strip assay based on a broad-specific monoclonal antibody (mAb) for simultaneous determination of CBF and 3-OH-CBF was developed. The colloidal gold-strips have a runtime within 5 min without complex sample pretreatment and perform a cut-off limit of detection (LOD) of 7–10 ng/mL for both carbofuran and 3-OH-CBF. The developed portable strip assay is a versatile and robust tool for the rapid and simultaneous detection of CBF and 3-OH-CBF in water samples as well as for the determination of illegal addition of CBF in other pesticide preparations.

中文翻译:

使用侧流免疫色谱法同时快速检测水样和农药制剂中的克百威和 3-羟基-克百威

摘要 开发快速鉴定和定量农药及其代谢物的方法是人们渴望已久的事情。克百威 (CBF) 及其代谢物 3-羟基-克百威 (3-OH-CBF) 对人类和非目标生物具有剧毒,这使得许多国家必须对其在食品和环境中的残留进行检测。在此,开发了一种基于广谱特异性单克隆抗体 (mAb) 的胶体金条检测方法,用于同时测定 CBF 和 3-OH-CBF。胶体金条的运行时间在 5 分钟内,无需复杂的样品预处理,对克百威和 3-OH-CBF 的检测截止限 (LOD) 为 7–10 ng/mL。
更新日期:2020-01-01
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