ABSTRACT Debregeasia orientalis, an important Chinese medicinal herb, has a long history for the treatment of rheumatic diseases. In this study, the antitumour activities of D. orientalis leaf polyphenols (DOLPs) against human cervical cancer Hela cells were investigated in vitro and in vivo. Hela cell proliferation, cell cycle, and apoptosis were investigated, the results revealed that DOLPs could inhibit Hela cell proliferation by blocking cell-cycle progression, inducing apoptosis and cell-cycle arrest. Moreover, Hela cells treated with DOLPs also showed an overproduction of reactive oxygen species (ROS) and a decrease of mitochondrial membrane potential. These results suggesting that the apoptotic effect of DOLPs on Hela cells was associated with an increased level of ROS and ROS production mediate apoptosis via the mitochondrial pathway. This article not only provides a basis for the functional mechanism and future application of DOLPs, but also promotes the high value utilization of D. orientalis leaf.

中文翻译：

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东方香叶中富含多酚的提取物在体外和体内对人宫颈癌的抑制作用

【摘要】：东香是一种重要的中草药,在治疗风湿病方面有着悠久的历史。在这项研究中，东方木叶多酚 (DOLPs) 对人宫颈癌 Hela 细胞的体外和体内抗肿瘤活性进行了研究。研究了 Hela 细胞增殖、细胞周期和细胞凋亡，结果表明 DOLPs 可以通过阻断细胞周期进程、诱导细胞凋亡和细胞周期停滞来抑制 Hela 细胞增殖。此外，用 DOLP 处理的 Hela 细胞也显示出活性氧 (ROS) 的过度产生和线粒体膜电位的降低。这些结果表明 DOLP 对 Hela 细胞的凋亡作用与 ROS 水平的增加有关，并且 ROS 的产生通过线粒体途径介导细胞凋亡。