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Development of an immunochromatographic lateral flow strip test for the rapid detection of diclofenac in medicinal wine
Food and Agricultural Immunology ( IF 3 ) Pub Date : 2020-01-01 , DOI: 10.1080/09540105.2020.1712331
Xingdong Yang 1, 2 , Yinbiao Wang 3 , Jifei Yang 2 , Zhongke Sun 1 , Cuiwei Chu 1 , Zonghao Yue 1 , Lili Li 1 , Xiaofei Hu 2
Affiliation  

ABSTRACT A rapid immunochromatographic lateral flow strip test (ILFST) of competitive format for the detection of diclofenac (DCF) in medicinal wine samples was developed. The half-inhibitory concentration (IC50) values of the ILFST for DCF and the limit of detection (LOD) in DCF-spiked samples were 0.66 and 0.15 ng/mL, respectively. Recoveries of DCF from spiked medicinal wine samples were from 84.4% to 85.9% within an assay (intra-assay) and from 83.7% to 88.4% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 4.10–7.56% and 4.71–8.94%, respectively. These results indicated that the ILFST was highly sensitive and re-producible. The same authentic medicinal wine samples were tested by both ILFST and high-performance liquid chromatography (HPLC) respectively. The results showed no significant differences between the two methods. Therefore, the ILFST can be widely used as a convenient tool for both qualitative and quantitative detection of DCF residues in medicinal wine samples.

中文翻译:

一种快速检测药酒中双氯芬酸的免疫层析侧流试纸检测方法

摘要 开发了一种用于检测药酒样品中双氯芬酸 (DCF) 的竞争形式的快速免疫层析侧向流条测试 (ILFST)。DCF 的 ILFST 半抑制浓度 (IC50) 值和 DCF 加标样品中的检测限 (LOD) 分别为 0.66 和 0.15 ng/mL。从加标药用酒样品中提取的 DCF 回收率在一次测定(测定内)内为 84.4% 至 85.9%,测定之间(测定间)为 83.7% 至 88.4%。批内和批间的变异系数 (CV) 分别为 4.10–7.56% 和 4.71–8.94%。这些结果表明 ILFST 是高度敏感和可重复的。分别通过ILFST和高效液相色谱(HPLC)测试相同的正宗药酒样品。结果表明两种方法之间没有显着差异。因此,ILFST 可广泛用作药用酒样品中 DCF 残留量定性和定量检测的便捷工具。
更新日期:2020-01-01
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