ABSTRACT The medicinal properties and toxicity of aconitine in traditional Chinese medicine have caused widespread concern. In this study, we screened a highly specific and sensitive monoclonal antibody with an IC50 of 3.65 ng/mL generated by immunizing mice and cell fusion, and the limits of detection of the ic-ELISA were 1.13 and 11.76 ng/mL. We then developed a colloidal gold test strip based on the antibody. For rapid detection of aconitine in herbs, the visual limit and detection limit of the test strip are 10 and 25 ng/mL, respectively; the results are obtained within 5 min and are visible to the naked eye. Therefore, the colloidal gold test strip is very suitable for on-site detection and quality screening of aconitine in Chinese herbal medicine.

中文翻译：

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用于检测乌头碱的 ic-ELISA 和免疫层析试纸条试验的开发

摘要 乌头碱在中药中的药性和毒性引起了广泛关注。本研究筛选出一种高特异性、高灵敏度的单克隆抗体，免疫小鼠和细胞融合产生的IC50为3.65 ng/mL，ic-ELISA检测限分别为1.13和11.76 ng/mL。然后我们开发了一种基于抗体的胶体金试纸。药材中乌头碱的快速检测，试纸的目测限和检出限分别为10和25 ng/mL；结果可在 5 分钟内获得，肉眼可见。因此，胶体金试纸非常适用于中草药中乌头碱的现场检测和质量筛选。