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High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system
Food and Agricultural Immunology ( IF 3 ) Pub Date : 2020-01-01 , DOI: 10.1080/09540105.2020.1713054
Yuan Li 1, 2 , Yahui Wang 1, 2, 3 , PeiPei Li 1, 2 , Yingjie Zhang 4 , Yuebin Ke 5 , Wenbo Yu 1, 2 , Kai Wen 1, 2 , Shuangyang Ding 1, 2 , Zhanhui Wang 1, 2 , Xuezhi Yu 1, 2
Affiliation  

ABSTRACT A simple, sensitive and specific chemiluminescence enzyme immunoassay based on biotin–streptavidin system (BASA-CLEIA) for diclazuril was established, which was about 2 and 4 times more sensitive than the traditional chemiluminescence enzyme immunoassay (CLEIA) and enzyme-linked immunosorbent assay. In the optimized generic assay, the half maximal inhibitory concentration (IC50) of the BASA-CLEIA for diclazuril was 0.48 μg/kg. The limit of detection for diclazuril was 0.02 μg/kg. This method showed no cross reactivity (<0.01%) with toltrazuril, robenidine hydrochloride, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. In chicken muscles, the average recoveries ranged from 90.3% to 120.4%. A further comparison between the developed BSAS-CLEIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for real samples showed good correlation (p < 0.01). The results indicated that the simple, sensitive and specific BASA-CLEIA method could be used for the analysis of diclazuril in real samples.

中文翻译:

基于生物素-链霉亲和素系统的鸡肌肉中地克珠利的高效化学发光免疫分析

摘要 建立了一种基于生物素-链霉亲和素系统 (BASA-CLEIA) 的简单、灵敏、特异的地克珠利化学发光酶免疫测定法,其灵敏度是传统化学发光酶免疫法 (CLEIA) 和酶联免疫吸附测定法的 2 和 4 倍左右。 . 在优化的通用试验中,BASA-CLEIA 对地克珠利的半数最大抑制浓度 (IC50) 为 0.48 μg/kg。地克珠利的检测限为 0.02 μg/kg。该方法与托曲珠利、盐酸罗苯尼定、尼卡巴嗪、卤夫酮、氨丙啉、莫能菌素和马杜霉素没有交叉反应性 (<0.01%)。在鸡肌肉中,平均恢复率为 90.3% 至 120.4%。对实际样品开发的 BSAS-CLEIA 和高效液相色谱-串联质谱 (HPLC-MS/MS) 方法的进一步比较显示出良好的相关性 (p < 0.01)。结果表明,该方法简单、灵敏、特异,可用于实际样品中地克珠利的分析。
更新日期:2020-01-01
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