ABSTRACT The development of highly selective analytical methodologies to monitoring phthalates (phthalic acid esters, PAEs) is important for the safety of environment, food and equipment. As one of the most important and classical interactions in biological systems, antigen–antibody interactions constitute a highly specific chemical nature in biological systems, which inspire us to develop highly specific, highly selective and high-throughput immunoassays for monitoring PAEs in different fields. We here attempt to give an introduction of the recent development particularly focusing on the immunoassay based on the specific binding of antigen–antibody for detecting PAEs. We also present an overview of the potential applications of the antibody labelling strategies to immunoassays for PAE investigations. Finally, we present the major challenges and opportunities regarding the high selectivity and specificity of PAE analysis based on the antigen–antibody interaction. We believe that this review provides critical insights into highly selective PAE analysis and gives an understanding of immunoassays.

中文翻译：

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测定邻苯二甲酸酯的免疫分析法的发展

摘要 开发用于监测邻苯二甲酸酯（邻苯二甲酸酯，PAE）的高选择性分析方法对于环境、食品和设备的安全非常重要。作为生物系统中最重要和最经典的相互作用之一，抗原-抗体相互作用构成了生物系统中高度特异性的化学性质，这激励我们开发高度特异性、高选择性和高通量的免疫分析来监测不同领域的 PAE。我们在此尝试介绍最近的发展，特别关注基于抗原 - 抗体特异性结合检测 PAE 的免疫分析。我们还概述了抗体标记策略在 PAE 研究免疫分析中的潜在应用。最后，我们提出了基于抗原 - 抗体相互作用的 PAE 分析的高选择性和特异性的主要挑战和机遇。我们相信这篇综述提供了对高度选择性 PAE 分析的重要见解，并提供了对免疫测定的理解。