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Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues
Food and Agricultural Immunology ( IF 3 ) Pub Date : 2020-01-01 , DOI: 10.1080/09540105.2020.1733496
Yong Xie 1 , Qiming Kou 2 , Qi Sun 2 , Yarong Wang 2 , Yi Cao 1 , Tao Le 2
Affiliation  

ABSTRACT Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled monoclonal antibody for the detection of pyrimethanil. Under optimal conditions, the visual cut-off value of ICTS for pyrimethanil was 40 ng/mL. Using a portable strip reader, the 50% inhibition concentration and calculated LOD of pyrimethanil in the PBS samples were 4.8 and 0.9 ng/mL, respectively. When used to analyse fortified samples of fruit and vegetable, acceptable recovery rates of 83.3–105.7% with the coefficient of variation values below 11.7% were obtained. Analysis of blindly fortified samples by the proposed ICTS gave similar results to those of the ic-ELISA and HPLC-MS/MS methods. These results indicate the proposed ICTS is a rapid, simple analytical method for quantitative on-site monitoring of pyrimethanil in foodstuffs.

中文翻译:

嘧霉胺残留快速检测免疫层析试纸条的研制与验证

摘要 嘧霉胺是一种广泛用于作物保护的苯胺基嘧啶类杀菌剂,常见于多种作物,包括水果、蔬菜和谷物。本文介绍了第一个报道的基于胶体金标记的单克隆抗体的免疫层析试纸条 (ICTS) 的开发,用于检测嘧霉胺。在最佳条件下,嘧霉胺的 ICTS 视觉截断值为 40 ng/mL。使用便携式条带读数器,PBS 样品中嘧霉胺的 50% 抑制浓度和计算 LOD 分别为 4.8 和 0.9 ng/mL。当用于分析水果和蔬菜的强化样品时,可接受的回收率为 83.3-105.7%,变异系数值低于 11.7%。建议的 ICTS 对盲目强化样品的分析给出了与 ic-ELISA 和 HPLC-MS/MS 方法相似的结果。这些结果表明,所提议的 ICTS 是一种快速、简单的分析方法,可对食品中的嘧霉胺进行现场定量监测。
更新日期:2020-01-01
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