The aim of this study is to determine the effect of GA on maize seedlings as well as its antioxidative effect under copper (Cu) stress. To that end, maize seedlings were exposed to 1.5 mM GA and 1 mM Cu solutions, and the seedlings were allocated to following experimental groups: 18-hr distilled water (DI) (Control), 6-hr GA + 12-hr DI (GA), 6-hr DI + 12-hr Cu (Cu), 6-hr GA + 12-hr Cu (GA + Cu), 12-hr Cu +6-hr GA (Cu + GA), and 18-hr GA and Cu mixture (GA = Cu). The experiment was designed by randomized block design with three repetitions. The results showed that GA alone and GA + Cu treatments significantly decreased membrane damage and H₂O₂ formation compared to the other treatments. GA formation was determined to be highest in turn in treatments GA + Cu and GA alone. The proline content significantly decreased in treatments GA alone and GA + Cu. The highest catalase (CAT) activity was observed in GA alone application, whereas GA + Cu treatment led to increase in guaiacol peroxidase (GPX), superoxide dismutase (SOD), and ascorbate peroxidase (APX) activities. GA has a protective effect on chlorophyll contents and relative water content (RWC), but not on carotenoid levels under Cu stress. Overall, GA alone application or GA + Cu stress may prevent the formation of reactive oxygen species (ROS) due to chelation effect of GA.

这项研究的目的是确定GA对玉米幼苗的影响及其在铜（Cu）胁迫下的抗氧化作用。为此，将玉米幼苗暴露于1.5 mM GA和1 mM Cu溶液中，并将幼苗分配到以下实验组中：18小时蒸馏水（DI）（对照），6小时GA + 12小时DI（ GA），6小时DI + 12小时Cu（Cu），6小时GA + 12小时Cu（GA + Cu），12小时Cu +6小时GA（Cu + GA）和18小时GA和Cu的混合物（GA = Cu）。该实验是通过随机重复三次重复的区块设计来设计的。结果表明，单独的GA和GA + Cu处理可显着降低膜损伤和H ₂ O ₂与其他处理相比。在单独的GA + Cu和GA处理中，GA形成被确定为最高。单独使用GA和GA + Cu时，脯氨酸含量显着下降。单独使用GA时观察到最高的过氧化氢酶（CAT）活性，而GA + Cu处理导致愈创木酚过氧化物酶（GPX），超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性增加。GA对叶绿素含量和相对水含量（RWC）具有保护作用，但对Cu胁迫下的类胡萝卜素水平无保护作用。总体而言，单独施用GA或GA + Cu胁迫可防止由于GA的螯合作用而形成活性氧（ROS）。