Although high strain and strain-rate impacts to the human body have been the subject of substantial research at both the systemic and tissue levels, little is known about the cell-level ramifications of such assaults. This is largely due to the lack of high throughput, dynamic compression devices capable of simulating such traumatic loading conditions on individual cells. To fill this gap, we developed and characterized a high speed, high actuation force, magnetically driven MEMS chip to apply stress to biological cells at unprecedented strain (10% to 90%), strain rate (30,000 to 200,000 s−1), and throughput (12,000 cells/min). To demonstrate the capabilities of the $\mu $ Hammer, we applied biologically relevant strains and strain rates to human leukemic K562 cells and then monitored their viability for up to 8 days. We observed significantly repressed proliferation of the hit cells compared to both unperturbed and sham-hit control cells, accompanied by minimal cell death. This indicates success in applying cellular damage without compromising the overall viability of the population, allowing us to conclude that this device is well suited to study the subtle effects of impact on large populations of inherently heterogeneous cells. [2019-0132]

中文翻译：

---

使用微流体 MEMS 设备研究细胞对撞击的响应

尽管对人体的高应变和应变率影响一直是系统和组织水平上大量研究的主题，但对此类攻击的细胞水平影响知之甚少。这主要是由于缺乏能够模拟单个细胞上这种创伤性负载条件的高通量、动态压缩设备。为了填补这一空白，我们开发并表征了一种高速、高驱动力、磁驱动的 MEMS 芯片，以前所未有的应变（10% 至 90%）、应变率（30,000 至 200,000 s-1）向生物细胞施加压力，并且吞吐量（12,000 个细胞/分钟）。为了证明 $\mu $ Hammer 的功能，我们将生物学相关的菌株和应变率应用于人类白血病 K562 细胞，然后监测它们的活力长达 8 天。我们观察到与未受干扰的和假击中的对照细胞相比，击中细胞的增殖受到显着抑制，伴随着最少的细胞死亡。这表明在不影响种群整体活力的情况下成功应用细胞损伤，使我们能够得出结论，该设备非常适合研究对大量固有异质细胞产生的影响的微妙影响。[2019-0132]