Background

Diabetes mellitus is characterized by hyperglycemia which displays insufficiency or resistance to insulin. One of the complications of diabetes is the increased risk of fracture and the impairment of bone repair and regulation. There have been evidences from previous studies that mesenchymal stem cells (MSCs) from bone marrow promote cartilage and callous formation. In addition, IL-10, an anti-inflammatory cytokine, has been observed to relieve inflammation-related complications in diabetes.

Methods

In this study, the role of IL-10-overexpressing bone marrow-derived MSCs (BM-MSCs) was examined in the diabetic mice model with femur fracture. MSCs were isolated from the BALB/c mice and IL-10 over expression was conducted with lentivirus transduction. The streptozotocin (STZ)-induced diabetes model with femoral fracture was established. BM-MSCs with IL-10 over expression were transplanted into the fracture area. The expressions of inflammatory factors IL-6, TNF-α and INF-γ were examined by qPCR and immunoblot; the biomechanical strength of the fracture site of the mice was examined and evaluated.

Results

Data showed that IL-10 overexpressed BM-MSCs transplantation decreased inflammatory response, promoted bone formation, and increased the strength of the fracture site in STZ-induced diabetic mice with femoral fracture.

Conclusion

IL-10 overexpressed BM-MSCs transplantation accelerated fracture repair in STZ-induced diabetic mice, which in turn provides potential clinical application prospects.

中文翻译：

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移植过表达 IL-10 的骨髓来源的间充质干细胞可改善糖尿病引起的小鼠骨折愈合障碍。

背景

糖尿病以高血糖为特征，表现为胰岛素不足或抵抗。糖尿病的并发症之一是骨折风险增加以及骨修复和调节受损。先前的研究有证据表明，来自骨髓的间充质干细胞 (MSCs) 可促进软骨和骨痂形成。此外，已观察到抗炎细胞因子 IL-10 可缓解糖尿病中与炎症相关的并发症。

方法

在这项研究中，在患有股骨骨折的糖尿病小鼠模型中检查了过表达 IL-10 的骨髓来源的 MSCs (BM-MSCs) 的作用。从 BALB/c 小鼠中分离出 MSC，并用慢病毒转导进行 IL-10 过表达。建立链脲佐菌素（STZ）诱导的糖尿病合并股骨骨折模型。将IL-10过表达的BM-MSCs移植到骨折区域。qPCR和免疫印迹检测炎症因子IL-6、TNF-α和INF-γ的表达；对小鼠骨折部位的生物力学强度进行了检查和评估。

结果

数据显示，IL-10 过表达的 BM-MSCs 移植可降低 STZ 诱导的股骨骨折糖尿病小鼠的炎症反应，促进骨形成，并增加骨折部位的强度。

结论

IL-10过表达的BM-MSCs移植加速了STZ诱导的糖尿病小鼠的骨折修复，这反过来又提供了潜在的临床应用前景。