Glutaric acid is an important organic acid applied widely in different fields. Most previous researches have focused on the production of glutaric acid in various strains using the 5-aminovaleric acid (AMV) or pentenoic acid synthesis pathways. We previously utilized a five-step reversed adipic acid degradation pathway (RADP) in Escherichia coli BL21 (DE3) to construct strain Bgl146. Herein, we found that malonyl-CoA was strictly limited in this strain, and increasing its abundance could improve glutaric acid production. We, therefore, constructed a malonic acid uptake pathway in E. coli using matB (malonic acid synthetase) and matC (malonic acid carrier protein) from Clover rhizobia. The titer of glutaric acid was improved by 2.1-fold and 1.45-fold, respectively, reaching 0.56 g/L and 4.35 g/L in shake flask and batch fermentation following addition of malonic acid. Finally, the highest titer of glutaric acid was 6.3 g/L in fed-batch fermentation at optimized fermentation conditions.

戊二酸是一种重要的有机酸，广泛应用于不同领域。以前的大多数研究都集中在使用5-氨基戊酸（AMV）或戊烯酸合成途径在各种菌株中生产戊二酸。我们先前利用大肠杆菌BL21（DE3）中的五步反向己二酸降解途径（RADP ）来构建菌株Bgl146。在此，我们发现丙二酰辅酶A在该菌株中受到严格限制，增加其丰度可以提高戊二酸的产量。因此，我们使用三叶草根瘤菌的matB（丙二酸合成酶）和matC（丙二酸载体蛋白）在大肠杆菌中构建了丙二酸摄取途径。戊二酸的滴度分别提高了2.1倍和1.45倍，在摇瓶和添加丙二酸后的分批发酵中分别达到0.56 g / L和4.35 g / L。最后，在优化的发酵条件下，分批补料发酵的戊二酸最高滴度为6.3 g / L。