In this study, we constructed a coculture consortium comprising engineered Pseudomonas putida KT2440 and Escherichia coli MG1655. Provision of “related” carbon sources and synthesis of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were separately assigned to these strains via a modular construction strategy. To avoid growth competition, a preference for the use of a carbon source was constructed. Further, the main intermediate metabolite acetate played an important role in constructing the expected “nutrition supply–detoxification” relationship between these strains. The coculture consortium showed a remarkable increase in the mcl-PHA titer (0.541 g/L) with a glucose–xylose mixture (1:1). Subsequently, the titer of mcl-PHA produced by the coculture consortium when tested with actual lignocellulosic hydrolysate (0.434 g/L) was similar to that achieved with laboratory sugars’ mixture (0.469 g/L). These results indicate a competitive potential of the engineered E. coli–P. putida coculture consortium for mcl-PHA production with lignocellulosic hydrolysate.

在这项研究中，我们建立了由工程假单胞菌假单胞菌KT2440和大肠杆菌组成的共培养联合体MG1655。通过模块化构建策略，将“相关的”碳源的提供和中链长度的聚羟基链烷酸酯（mcl-PHA）的合成分别分配给了这些菌株。为了避免增长竞争，人们提出了使用碳源的偏好。此外，主要的中间代谢产物乙酸盐在构建这些菌株之间预期的“营养供应-解毒”关系方面发挥了重要作用。共培养联合体显示，使用葡萄糖-木糖混合物（1：1）时，mcl-PHA滴度（0.541 g / L）显着增加。随后，当用实际的木质纤维素水解产物（0.434 g / L）测试时，由共培养财团生产的mcl-PHA的效价类似于用实验室糖的混合物（0.469 g / L）实现的效价。这些结果表明了工程技术人员的竞争潜力。大肠杆菌–恶臭假单胞菌共培养财团，用于利用木质纤维素水解产物生产mcl-PHA。