This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS intensity showed a good linear relationship with TNF-α and IL-1β concentrations in our study. The results of detection of these biomarkers in GCF indicate excellent specificity, high sensitivity, and great reproducibility of this immunoassay. Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases.

中文翻译：

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基于表面增强拉曼散射的牙周疾病人牙龈沟液中两种细胞因子的免疫测定

这项研究结合了拉曼技术和纳米技术来检测牙周炎性因子。我们合成了银纳米颗粒，并对它们的表面进行了修饰，以生产Ag NP-抗体探针。开发了一种夹心免疫测定法，用于灵敏检测TNF-α和IL-1β。在我们的研究中，SERS强度与TNF-α和IL-1β的浓度表现出良好的线性关系。在GCF中检测这些生物标志物的结果表明该免疫测定法具有出色的特异性，高灵敏度和高可重复性。我们基于SERS技术的免疫分析将有助于牙周疾病的临床诊断。