Abstract

On the basis of the data of infrared spectroscopy with synchrotron radiation, the secondary structure of proteins of the dentinal and gingival fluids during the development of cariosity in deep dentin tissues is studied. It is shown that the change in the shape of the profile of the amide I band in the region of 1700‒1605 cm^–1 is associated both with a change in the ratio of the integrated absorption intensities of the α‑helix and β-sheet secondary structures and with the position of the β-coil and β-sheet components in the spectrum. It is established that the α-helix/β-sheet ratio for both dentinal and gingival fluids is below the threshold level, at which significant changes in the secondary structure of proteins of biological fluids are observed, unequivocally indicating the development of pathology in hard dental tissues. The features that we discovered in the profile of the amide I band of biological fluids of the oral cavity, together with the spectral markers of the development of cariosity in dentin, are reliable spectroscopic signatures of the pathology and can be detected using the gingival fluid.

中文翻译：

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同步辐射红外光谱法研究口腔生物流体蛋白质二级结构的变化

摘要

基于同步加速器辐射的红外光谱数据，研究了深部牙本质组织龋齿形成过程中牙本质和牙龈液中蛋白质的二级结构。结果表明，在1700‒1605 cm ^–1范围内，酰胺I带的轮廓形状发生了变化。与α-螺旋和β-折叠二级结构的综合吸收强度之比的变化以及光谱中β-线圈和β-折叠成分的位置有关。已经确定，牙本质和牙龈液的α-螺旋/β-片层比率均低于阈值水平，在该阈值水平上观察到生物流体蛋白质的二级结构发生了显着变化，明确表明了硬牙病的病理学发展组织。我们在口腔生物液的酰胺I带谱中发现的特征，以及牙本质中龋齿发展的光谱标记，是病理学的可靠光谱特征，可以使用牙龈液进行检测。