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Superoxide Dismutase Activity in Phylogenetically Heterogeneous Regions of the Rat Brain during Repetitive Sessions of Hyperbaric Oxygenation
Journal of Evolutionary Biochemistry and Physiology ( IF 0.6 ) Pub Date : 2019-09-01 , DOI: 10.1134/s0022093019050089
Ya. V. Bulgakova , P. N. Savilov , V. N. Yakovlev , E. V. Dorokhov

Our study addresses the effect of repetitive sessions of hyperbaric oxygenation (HBO) (2 ATA, 50 minutes, 1 session a day) on superoxide dismutase (SOD) activity as assayed by a chemiluminescent method in the phylogenetically heterogeneous brain structures (brain stem, cerebellum, cerebral hemispheres) of white rats. A study of intact animals (series 1, control) revealed no statistically significant differences in SOD activity between these structures. One HBO session (series 2) stimulated SOD activity in all the brain structures: in the hemispheres, cerebellum and stem by 41, 31 and 66% vs. control, respectively (p < 0.05). After five HBO sessions (series 3), the SOD response intensity in the brain stem exceeded that in the other structures (p < 0.05) and increased by 187% vs. control (p < 0.001). Further continuation of oxygenation up to ten sessions (series 4) was accompanied by a normalization of SOD activity. After eighteen HBO sessions (series 5), the SOD activity level in the cerebellum and cerebral hemispheres somewhat increased compared to the 10th HBO session, although remained closer to the values of the control group than to those of the brain stem. In the brain stem, the SOD activity level exceeded that in intact rats by 46% (p < 0.05).

中文翻译:

重复高压氧作用期间大鼠脑系统发育异质区域的超氧化物歧化酶活性

我们的研究解决了重复性高压氧 (HBO)(2 ATA,50 分钟,每天 1 次)对超氧化物歧化酶 (SOD) 活性的影响,如通过系统发育异质脑结构(脑干、小脑)中的化学发光方法测定的,大脑半球)的白色大鼠。对完整动物(系列 1,对照)的研究表明,这些结构之间的 SOD 活性没有统计学上的显着差异。一次 HBO 会议(系列 2)刺激了所有大脑结构中的 SOD 活动:在半球、小脑和茎中,与对照组相比,分别高出 41%、31% 和 66%(p < 0.05)。在五次 HBO 会议(系列 3)之后,脑干中的 SOD 反应强度超过了其他结构中的(p < 0.05),并且比对照增加了 187%(p < 0.001)。氧合的进一步持续到十个疗程(系列 4)伴随着 SOD 活性的正常化。在 18 个 HBO 会话(系列 5)之后,小脑和大脑半球的 SOD 活动水平与第 10 个 HBO 会话相比有所增加,尽管与脑干的值相比仍更接近对照组的值。在脑干中,SOD 活性水平超过完整大鼠的 46% (p < 0.05)。
更新日期:2019-09-01
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