Abstract

Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the length of the growing mycelium) with the concentrations of antigens detected by the two-site sandwich ELISA method and the competitive ELISA method was evaluated. It has been shown that the methods are highly effective in the case of long incubation (more than 5 days) of soil samples and are ineffective for short-term incubation, because there was no correlation between hypha length and antigen concentration during the first day of cultivation. The perspectivity of ELISA methods for the detection of some soil microfungi from genera Aspergillus (Asp. flavus, Asp. fumigatus, Asp. niger), Fusarium (F. solani, F. poae), and others (Alternaria alternata, Phoma lingham, Mucor hiemalis) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA.

中文翻译：

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酶联免疫吸附法测定土壤提取物中许多微真菌的菌丝体和抗原

摘要

研究了十六种测试系统的变体（两点三明治和竞争性酶联免疫吸附测定（ELISA））以确定土壤提取物中微真菌的浓度，其中一些是已知的人为机会性病原体。评估了真菌生物量的量（生长的菌丝体的长度）与通过两点夹心ELISA方法和竞争性ELISA方法检测到的抗原浓度的相关性。结果表明，该方法在土壤样品长时间孵育（超过5天）时非常有效，而对于短期孵育则无效，这是因为在培养的第一天，菌丝长度和抗原浓度之间没有相关性。培养。ELISA方法检测某些属土壤微真菌的透视曲霉（天冬氨酸。黄曲霉，是Asp。曲霉，是Asp。曲霉），镰孢属（茄病镰刀菌，F. poae），和其他（链格孢，茎点lingham，冻土毛霉）中的溶液中。竞争性ELISA方法比两点夹心ELISA更有效。