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Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees
Apidologie ( IF 2.4 ) Pub Date : 2020-03-16 , DOI: 10.1007/s13592-020-00747-4
Jerzy Paleolog , Jerzy Wilde , Maciej Siuda , Beata Bąk , Łukasz Wójcik , Aneta Strachecka

Imidacloprid (IMD) may affect proteolysis, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and global DNA methylation in honeybees. Queens, drones, and workers aged 1 or 20 days were exposed (free-flying colonies) to IMD (5 ppb and 200 ppb) in their diet. As a result, the colony depopulation did not occurred. IMD disturbed hemolymph/cuticle proteolysis; deactivated most of the cuticle protease inhibitors, activated hemolymph thiol and metal proteases and cuticle thiol proteases; downregulated ALP, ALT, AST; and increased DNA methylation in a caste- and age-dependent manner. The response in queens and workers differed, possibly due to eusocial evolution. Higher IMD dose had greater effects. The responses of ALP, ALT, AST, and DNA may reflect acceleration of biochemical senescence and epigenetic adaptation to IMD. All these biochemical side effects may lead to colony depopulation during future biotic/abiotic stress.

中文翻译:

吡虫啉显着影响西方蜜蜂的血淋巴蛋白水解、生物标志物、DNA 整体甲基化和角质层蛋白水解层

吡虫啉 (IMD) 可能会影响蜜蜂的蛋白质水解、天冬氨酸氨基转移酶 (AST)、丙氨酸氨基转移酶 (ALT)、碱性磷酸酶 (ALP) 和整体 DNA 甲基化。蜂王、雄蜂和 1 天或 20 天大的工蚁在其饮食中暴露于(自由飞行的群体)IMD(5 ppb 和 200 ppb)。结果,没有发生菌落人口减少。IMD 扰乱血淋巴/角质层蛋白水解;使大多数角质层蛋白酶抑制剂、活化的血淋巴硫醇和金属蛋白酶以及角质层硫醇蛋白酶失活;ALP、ALT、AST 下调;并以种姓和年龄依赖性方式增加 DNA 甲基化。蜂后和工蜂的反应不同,可能是由于社会进化。更高的 IMD 剂量有更大的影响。ALP、ALT、AST 的反应,DNA 可能反映生化衰老和表观遗传适应 IMD 的加速。所有这些生化副作用都可能导致未来生物/非生物胁迫期间菌落数量减少。
更新日期:2020-03-16
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