Phoretic mites associated with bumblebees are considered commensals that represent little or no risk for their hosts. The aim of the present study was to assess the potential role of the phoretic mites Pneumolaelaps longanalis and Parasitellus fucorum, as carriers of parasites that are known to affect bees. Mites were extracted from bumblebees dating between 1945 and 1981 which belong to museum collections, and from a freshly collected queen. The DNA was extracted and amplified, and the final products were sequenced and analyzed. Parasite’s DNA was found in all samples. Ascosphaera spp., Crithidia bombi, Lotmaria passim, and Nosema ceranae were present in both mite species. Moreover, DNA from Apis mellifera filamentous virus (AmFV), Apicystis bombi, Ascosphaera apis, Nosema apis, and Nosema bombi was found in P. longanalis. This study represents the oldest report of parasite’s DNA in bumblebee phoretic mites, highlighting the relevance of museum samples for historical studies in parasitology.

中文翻译：

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南美大黄蜂（Bombus spp.）上的蠕虫作为寄生虫载体：历史输入

与大黄蜂相关的蠕虫被认为是对宿主几乎没有风险的共生体。本研究的目的是评估蠕虫 Pneumolaelaps longanalis 和 Parasitellus fucorum 作为已知会影响蜜蜂的寄生虫载体的潜在作用。螨虫是从 1945 年至 1981 年间属于博物馆收藏的大黄蜂和新收集的蜂王中提取的。提取并扩增DNA，并对最终产物进行测序和分析。在所有样本中都发现了寄生虫的 DNA。Ascosphaera spp.、Crithidia bombi、Lotmaria passim 和 Nosema ceranae 存在于两种螨类中。此外，在 P. longanalis 中发现了来自蜜蜂丝状病毒 (AmFV)、Apicystis bombi、Ascosphaera apis、Nosema apis 和 Nosema bombi 的 DNA。