Transient receptor potential (TRP) channels are critical for insects to detect environmental stimuli and regulate homeostasis. Moreover, this superfamily has become potential molecular targets for insecticides or repellents. Pieris rapae is one of the most common and widely spread pests of Brassicaceae plants. Therefore, it is necessary to study TRP channels (TRPs) in P. rapae. In this study, we identified 14 TRPs in P. rapae, including two Water witch (Wtrw) genes. By contrast, only one Wtrw gene exists in Drosophila and functions in hygrosensation. We also found splice isoforms of Pyrexia (Pyx), TRPgamma (TRPγ) and TRP-Melastatin (TRPM). These three genes are related to temperature and gravity sensation, fine motor control, homeostasis regulation of Mg²⁺ and Zn²⁺ in Drosophila, respectively. Evolutionary analysis showed that the TRPs of P. rapae were well clustered into their own subfamilies. Real-time quantitative PCR (qPCR) showed that PrTRPs were widely distributed in the external sensory organs, including antennae, mouthparts, legs, wings and in the internal physiological organs, including brains, fat bodies, guts, Malpighian tubules, ovaries, as well as testis. Our study established a solid foundation for functional studies of TRP channels in P. rapae, and would be benefit to developing new approaches to control P. rapae targeting these important ion channels.

中文翻译：

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蔬菜害虫菜青虫中TRP通道基因的鉴定，表征和表达分析

瞬态受体电位（TRP）通道对于昆虫检测环境刺激和调节体内稳态至关重要。而且，该超家族已经成为杀虫剂或驱虫剂的潜在分子靶标。菜青虫是十字花科植物中最常见和广泛传播的害虫之一。因此，有必要研究油菜假单胞菌中的TRP通道（TRPs）。在这项研究中，我们确定了14个TRP的菜粉蝶，其中包括两名巫水（Wtrw）基因。相比之下，果蝇a中仅存在一个Wtrw基因，并具有增湿作用。我们还发现的剪接异构体热病（PYX）TRPgamma（TRPγ）和TRP-Melastatin（TRPM）。这三个基因都与温度和重力感，细调电机控制，镁的稳态调节²⁺和Zn ²⁺中果蝇，分别。进化分析表明，菜青虫的TRP很好地聚集在自己的亚科中 实时定量PCR（qPCR）显示，PrTRPs广泛分布于外部感觉器官，包括触角，口器，腿，翅膀和内部生理器官，包括脑，脂肪体，胆量，马尔皮基小管，卵巢。作为睾丸。我们的研究为菜青虫中TRP通道的功能研究奠定了坚实的基础，并将有助于开发针对这些重要离子通道的控制菜青虫的新方法。