In Sri Lanka, dengue is the most serious arboviral disease. Recent increases in dengue cases suggest a higher infection rate and spread of the disease to new areas. The present study explores gene flow patterns of Ae. aegypti, the main vector of dengue disease, among 10 collection sites including major ports and inland cities using variations at 11 microsatellite loci. Discriminant analysis of principal components (DAPC) and k-means clustering estimated eight genetic clusters. Analysis of Molecular Variance (AMOVA) estimated equal variances among cities and among collections in Colombo, Sri Lanka. Significant evidence, although weak, was detected for isolation by distance. Analysis of gene flow rates and directions using MIGRATE-n indicated that populations throughout the island served as a source of immigrants for Colombo with abundant gene flow among major commercial cities in Sri Lanka, which appear to receive migrant mosquitoes from throughout Sri Lanka. The observed patterns probably arise through human movement of Ae. aegypti during commerce from throughout Sri Lanka into Colombo increasing the risk of spread. The patterns uncovered in this study are significant for global health as Sri Lanka is situated along a key international shipping route.

中文翻译：

---

斯里兰卡埃及伊蚊（双翅目：蚊科）种群的基因流模式

在斯里兰卡，登革热是最严重的虫媒病毒疾病。最近登革热病例的增加表明感染率较高并且该疾病已传播到新的地区。本研究探讨了伊蚊的基因流模式。埃及伊蚊是登革热疾病的主要传播媒介，利用 11 个微卫星位点的变异，在包括主要港口和内陆城市在内的 10 个收集点中发现了埃及伊蚊。主成分判别分析 (DAPC) 和k均值聚类估计了 8 个遗传簇。分子方差分析 (AMOVA) 估计斯里兰卡科伦坡的城市之间和馆藏之间的方差相等。尽管证据薄弱，但仍发现了通过距离进行隔离的重要证据。使用 MIGRATE-n 对基因流动速率和方向进行的分析表明，整个岛上的人口是科伦坡的移民来源，在斯里兰卡的主要商业城市之间有丰富的基因流动，这些城市似乎接收了来自斯里兰卡各地的迁徙蚊子。观察到的模式可能是通过人类的伊蚊运动而产生的。从斯里兰卡各地进入科伦坡的商业活动中，埃及伊蚊增加了传播风险。由于斯里兰卡位于重要的国际航运路线沿线，本研究发现的模式对全球健康具有重要意义。