Abstract

Background

Although listed in the Stockholm Convention, commercial Decabromodiphenyl ether (c-DecaBDE) is still being produced in many factories and used as a kind of flame retardants primarily in plastic polymers and textiles. Widespread use offered many exposure ways of its major ingredient, BDE-209, to humans and the environment. Most current studies of BDE-209 focused on the health effects and toxicity of thyroid disruption, oxidative stress, neurotoxicity, and reproductive function, but seldom spread light on the relationship between neurobehavioral toxicity and visual dysfunction. Using zebrafish larvae model, we hope to uncover the potential relationship between the neurobehavioral and visual effects after exposure to BDE-209.

Results

BDE-209 exposure could not induce the changes of locomotion and path angle in 5 days post fertilization (dpf) larvae; however, 5 μg/L BDE-209 exposure caused locomotor hyperactivity and more responsive turns at 7 dpf. The social activity of 50 μg/L exposure group was significantly higher than the control group at 6 dpf. Besides, 5 and 50 μg/L exposure caused the upregulation and downregulation of four cone opsin genes, respectively. The expression of rhodopsin gene was not influenced by both concentration exposures.

Conclusion

The neurobehavioral effects induced by 5 μg/L BDE-209 exposure were consistent with the upregulation of four cone opsins in 7 dpf larvae. The low concentration of BDE-209 exposure caused the hyperactivity and more responsive turns of larvae possibly contributing to the disruption on the cone opsin expressions of larvae. Our results would provide the mechanism cue of neurobehavioral toxicity after BDE-209 exposure and call for more attention on the ecotoxicology studies of BDE-209.

中文翻译：

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BDE-209对斑马鱼幼虫神经行为毒性与视觉功能障碍之间的潜在关系：一项初步研究

摘要

背景

尽管被列入《斯德哥尔摩公约》，但商用十溴二苯醚（c-DecaBDE）仍在许多工厂中生产，并且主要用作塑料聚合物和纺织品中的一种阻燃剂。广泛使用其主要成分BDE-209对人体和环境有多种接触方式。BDE-209的最新研究集中在甲状腺功能紊乱，氧化应激，神经毒性和生殖功能的健康影响和毒性上，但很少阐明神经行为毒性和视功能障碍之间的关系。我们希望使用斑马鱼幼虫模型来揭示暴露于BDE-209后神经行为与视觉效果之间的潜在关系。

结果

BDE-209暴露在受精（dpf）幼虫后5天内不能引起运动和路径角的变化；但是，暴露于5μg/ L的BDE-209会引起运动过度活跃，在7 dpf时反应更加灵敏。50 dg / L暴露组的社交活动在6 dpf时显着高于对照组。此外，5和50μg/ L的暴露分别导致四个视锥蛋白基因的上调和下调。视紫红质基因的表达不受两次浓度暴露的影响。

结论

BDE-209暴露于5μg/ L诱导的神经行为与7 dpf幼虫中四个视锥蛋白的上调一致。低浓度的BDE-209暴露引起幼虫的活动过度和更敏感的转弯，可能导致幼虫视锥蛋白表达的破坏。我们的结果将为暴露BDE-209后神经行为毒性的机理提供线索，并呼吁更多地关注BDE-209的生态毒理学研究。