Conservation of the endangered freshwater pearl mussel (FPM) includes artificially rearing juveniles, but the pulsed flow-through (PFT) method, enabling the continuous renewal of water and food in culture containers, has not been applied to FPM. This study tested the PFT method in culture of FPM juveniles, and the effect of water source (tap vs well water) and food concentration (mixture of commercial phytoplankton products) on the survival and growth of juveniles. Beaker-specific survival rates varied from 0 to 100% (mean: 34%) and from 0 to 58% (mean: 16%) in the 1st (2-week) and 2nd (10-week) experiment, respectively. In the 1st experiment, juveniles attained statistically significantly bigger sizes and more than two times higher survival in well water than they did in tap water. In both experiments, the food concentration of 0.250 µl/l resulted in the highest survival (compared to other concentrations ranging from 0.125 to 0.500 µl/l). However, food concentration did not have a significant effect on size of juvenile FPM in either of the experiments. Results indicate that the PFT system is suitable for FPM rearing experiments, but its applicability to mass culture of FPM juveniles requires further investigation.

中文翻译：

---

Margaritifera margaritifera 脉冲流水栽培：水源和食量对幼鱼存活和生长的影响

濒危淡水珍珠贻贝 (FPM) 的保育包括人工饲养幼体，但脉冲流通 (PFT) 方法能够持续更新养殖容器中的水和食物，尚未应用于 FPM。本研究测试了 FPM 幼鱼养殖中的 PFT 方法，以及水源（自来水与井水）和食物浓度（商业浮游植物产品的混合物）对幼鱼存活和生长的影响。在第一次（2 周）和第二次（10 周）实验中，烧杯特异性存活率分别从 0 到 100%（平均：34%）和从 0 到 58%（平均：16%）不等。在第一个实验中，幼鱼在井水中获得了统计学上显着更大的体型和比在自来水中高两倍以上的存活率。在这两个实验中，食物浓度均为 0。250 µl/l 导致最高的存活率（与其他浓度范围从 0.125 到 0.500 µl/l 相比）。然而，在这两个实验中，食物浓度对幼鱼 FPM 的大小没有显着影响。结果表明 PFT 系统适用于 FPM 饲养实验，但其对 FPM 幼鱼的大规模培养的适用性需要进一步研究。