Abstract The development of biocompatible patches that can provide adequate mechanical properties and mimicking the physiological relevance still has enormous challenges in the field of tissue engineering. In this study, the aim is to engineer and develop tri-layered nanofiber patches to synchronous the mechanical properties and mimic the complex structure of living cardiovascular tissues. The developed patches consisting of blended silk fibrin (SF) and polyvinyl alcohol (PVA) composite as a hydrophilic middle layer. The upper and lower layers were composed of polycaprolactone polymer (PCL) and Poly (Lactic Acid) (PLA), respectively. Layer by layer of the developed patches were fabricated by electrospinning technique. The patches characteristics such as morphology, surface topography, and physiochemical, biodegradable and mechanical stability were investigated. Furthermore, a human endothelial cell line (EA. hy926) was exploited to assess the biological properties of the fabricated patches. The results show that the patches had high endothelial cells biocompatibility, mechanical elasticity, and unique nanofibers structure.

中文翻译：

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用于心脏组织工程的具有内皮细胞的生物相容性三层纳米纤维贴片的开发

摘要 开发能够提供足够机械性能并模拟生理相关性的生物相容性贴片在组织工程领域仍然面临巨大挑战。在这项研究中，目的是设计和开发三层纳米纤维贴片，以同步机械性能并模拟活体心血管组织的复杂结构。开发的贴片由混合丝纤维蛋白 (SF) 和聚乙烯醇 (PVA) 复合材料组成，作为亲水中间层。上层和下层分别由聚己内酯聚合物 (PCL) 和聚乳酸 (PLA) 组成。通过静电纺丝技术逐层制造开发的贴片。斑块的形态、表面形貌和理化等特征，研究了生物可降解性和机械稳定性。此外，利用人内皮细胞系（EA.hy926）来评估制造的贴片的生物学特性。结果表明，贴片具有较高的内皮细胞生物相容性、机械弹性和独特的纳米纤维结构。