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Proteomic profiling reveals the differential toxic responses of gills of common carp exposed to nanosilver and silver nitrate.
Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.jhazmat.2020.122562
Qian-Qian Xiang 1 , Ying Gao 1 , Qin-Qin Li 1 , Jian Ling 2 , Li-Qiang Chen 1
Affiliation  

Although the toxic effects of silver nanoparticles (AgNPs) on fish gills have been reported, the underlying mechanism of toxicity remains unclear. The present study aimed to elucidate the mechanism of toxicity in the gills of common carp following exposure to AgNPs and silver nitrate (AgNO3) using histopathology and proteomics. Histopathological findings revealed that both AgNPs and AgNO3 caused telangiectasia and epithelial cell hyperplasia in fish gills; however, the pathological features and location of lesions caused by the two forms of silver were markedly different. Proteomics revealed that AgNPs and AgNO3 induced 139 and 185 differential expression proteins (DEPs) in gills, respectively, and the two forms of silver induced only 42 shared proteins. AgNPs specifically induced 87 DEPs which mainly involved signaling mechanisms, cytoskeleton, and the arachidonic acid metabolism processes. AgNO3 specifically induced 125 DEPs that were mainly clustered in the glutathione metabolism and protease processes. These results suggested that the toxic effects of AgNPs and AgNO3 were dramatically different in terms of protein expression in fish gills, which may provide novel perspectives for understanding the toxicity mechanism of silver nanoparticles in fish gills.

中文翻译:

蛋白质组学分析揭示了暴露于纳米银和硝酸银的鲤鱼g的不同毒性反应。

尽管已经报道了银纳米颗粒(AgNPs)对鱼g的毒性作用,但其潜在的毒性机理仍不清楚。本研究旨在通过组织病理学和蛋白质组学阐明暴露于AgNPs和硝酸银(AgNO3)后鲤鱼the的毒性机制。组织病理学发现表明,AgNPs和AgNO3均可引起鱼g的毛细血管扩张和上皮细胞增生。然而,两种形式的银引起的病理特征和病变位置明显不同。蛋白质组学表明,AgNP和AgNO3分别诱导g中的139和185个差异表达蛋白(DEP),而两种形式的银仅诱导42种共有蛋白。AgNPs特异诱导了87种DEP,主要涉及信号传导机制,细胞骨架,和花生四烯酸的代谢过程。AgNO3特异性诱导了125种DEP,它们主要聚集在谷胱甘肽的代谢和蛋白酶过程中。这些结果表明,AgNPs和AgNO3的毒性作用在鱼g中的蛋白质表达方面有显着差异,这可能为了解银纳米颗粒在鱼g中的毒性机理提供新的观点。
更新日期:2020-03-20
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