当前位置: X-MOL 学术Ticks Tick Borne Dis. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Identification and validation of internal reference genes for real-time quantitative polymerase chain reaction-based studies in Hyalomma anatolicum ticks.
Ticks and Tick-Borne Diseases ( IF 3.2 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.ttbdis.2020.101417
Cristiano Salata 1 , Vanessa Monteil 2 , Mikael Leijon 3 , Lesley Bell-Sakyi 4 , Ali Mirazimi 5
Affiliation  

Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne viral disease caused by the orthonairovirus CCHF virus (CCHFV). Ticks of the genus Hyalomma are the viral reservoir and they represent the main vector transmitting the virus to their hosts during blood feeding. However, how CCHFV replicates in its natural arthropod host cells and the nature of virus/host interactions are still largely unknown. With the aim of developing tools for use in this field, we identified and validated expression of four candidate endogenous control tick genes in a Hyalomma anatolicum-derived cell line. These genes will be useful for normalization of viral/cellular transcripts in infection/expression studies or as internal controls in molecular epidemiology surveys of pathogens transmitted by Hyalomma ticks.



中文翻译:

鉴定和验证内部参比基因,用于实时定量聚合酶链节中基于定量定量聚合酶链反应的研究。

克里米亚-刚果出血热(CCHF)是一种正传传染的tick传病毒性疾病,由正正iroiroirovirus CCHF病毒(CCHFV)引起。Hyalomma的虫是病毒库,它们代表了在喂血过程中将病毒传播到宿主的主要载体。然而,CCHFV如何在其自然节肢动物宿主细胞中复制以及病毒/宿主相互作用的性质仍是未知之数。为了开发在该领域中使用的工具,我们鉴定并验证了四个透明质酸来源的细胞系中四个候选内源性控制tick基因的表达。这些基因可用于感染/表达研究中病毒/细胞转录本的正常化,或作为由分子传播的病原体的分子流行病学调查的内部对照透明膜tick虫。

更新日期:2020-03-19
down
wechat
bug