A novel chemiluminescence (CL) reaction was proposed to determine naproxen and naltrexone based on the synergistic effect of Chromotrope 2R in the CL system of acidic KMnO₄. Eight dyes including Chromotrope 2R, Rhodamine 6G, Amido black 10B, Eosin Y, Amaranth, Brilliant blue, Orange G and Victoria blue were evaluated, and Chromotrope 2R produced the highest signal and signal to background for the analytes, namely ascorbic acid, citric acid, oxalic acid, tartaric acid, maleic acid, thiourea, codeine, pholcodine, naproxen and naltrexone. Among these species, naproxen and naltrexone were chosen as model analytes. In the proposed method, the weak CL intensity of acidic KMnO₄-naoproxen (or other analytes) increased significantly in the presence of Chromotrope 2R, and the CL intensity was proportional to naproxen (or naltrexone) concentration. The CL reaction mechanism was investigated through UV–Vis, fluorescence and CL spectra. Under optimal conditions, the CL intensity was found to be linearly proportional with the naproxen and naltrexone concentrations respectively in the range of 23–4600 μg mL⁻¹ and 1.3–238.7 μg mL⁻¹. The limits of detection for naproxen and naltrexone were 4.7 and 0.1 μg mL⁻¹, respectively. The relative standard deviation (n = 7) for 161.2 μg mL⁻¹ naproxen and 13.6 μg mL⁻¹ naltrexone was 4.8% and 5.9%, respectively. This method was used for determination of naproxen in drug formulations and naltrexone in synthetic samples.

提出了一种新的化学发光（CL）反应，根据Chromotrope 2R在酸性KMnO ₄的CL体系中的协同作用来测定萘普生和纳曲酮。对八种染料进行了评估，其中包括Chromotrope 2R，若丹明6G，酰胺黑10B，曙红Y，A菜红，亮蓝，橙G和维多利亚蓝，并且Chromotrope 2R产生了最高的信号和背景信号，即抗坏血酸，柠檬酸，草酸，酒石酸，马来酸，硫脲，可待因，福尔可定，萘普生和纳曲酮。在这些物种中，选择萘普生和纳曲酮作为模型分析物。在所提出的方法中，酸性KMnO ₄的弱CL强度存在Chromotrope 2R时，萘普生（或其他分析物）显着增加，并且CL强度与萘普生（或纳曲酮）浓度成正比。通过UV-Vis，荧光和CL光谱研究了CL反应机理。在最佳条件下，化学发光强度被认为是与萘普生和纳曲酮的浓度成线性比例分别为23-4600微克mL范围内^-1和1.3-238.7微克毫升^-1。萘普生和纳曲酮的检出限分别为4.7和0.1μgmL ^-1。为161.2微克mL的相对标准偏差（n = 7）^-1萘普生和13.6微克毫升^-1纳曲酮分别为4.8％和5.9％。该方法用于测定药物制剂中的萘普生和合成样品中的纳曲酮。