Abstract The new ABTS-dot-blot method for the direct determination of antioxidant activity of active packaging that is in contact with foodstuffs has been developed. The usefulness of the new method was verified with the use of agarose, pork gelatin, bacterial cellulose and cellulose-chitosan films with incorporated standard antioxidant – Trolox or plant phytochemicals derived from three types of berry juices (chokeberry, blue-berried honeysuckle, rowanberry) or green tea. The plant components used for preparation of films were characterized by antioxidant profiling with the use of HPLC coupled with post-column derivatization with ABTS. The most abundant antioxidants were polyphenols, mainly anthocyanins, hydroxycinnamates and flavan-3-ols. The antioxidant properties of different types of films studied were evaluated by typical cuvette spectrophotometric ABTS test or by the novel method employing ABTS radical stabilised in an agarose gel. The results obtained for the Trolox containing films showed quantitative linear relationship between antioxidant content and the degree of ABTS bleaching in agarose gel. The results of both standard spectrophotometric method as well as ABTS-dot-blot approach indicated that gelatin, bacterial cellulose and chitosan on their own possessed antioxidant activity, which was substantially increased (6–10 times) by the addition of phytochemicals during film preparation. The highest antioxidant activity was observed for microbial cellulose films, which were prepared on the basis of material obtained during the kombucha drink production.

中文翻译：

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用于评估食品包装抗氧化性能的新型 ABTS 斑点印迹法

摘要 开发了一种新的 ABTS 斑点印迹法，用于直接测定与食品接触的活性包装的抗氧化活性。通过使用琼脂糖、猪肉明胶、细菌纤维素和纤维素-壳聚糖薄膜并加入标准抗氧化剂——Trolox 或源自三种浆果（苦莓、蓝浆果金银花、罗文莓）的植物植物化学物质，验证了新方法的有效性或绿茶。用于制备薄膜的植物成分通过使用 HPLC 与柱后衍生化与 ABTS 进行抗氧化分析来表征。最丰富的抗氧化剂是多酚，主要是花青素、羟基肉桂酸酯和黄烷-3-醇。通过典型的比色皿分光光度法 ABTS 测试或采用在琼脂糖凝胶中稳定的 ABTS 自由基的新方法来评估所研究的不同类型薄膜的抗氧化性能。对于含有 Trolox 的薄膜获得的结果显示抗氧化剂含量与琼脂糖凝胶中 ABTS 漂白程度之间的定量线性关系。标准分光光度法和 ABTS 斑点印迹法的结果表明，明胶、细菌纤维素和壳聚糖本身具有抗氧化活性，在薄膜制备过程中添加植物化学物质可显着提高（6-10 倍）。微生物纤维素薄膜的抗氧化活性最高，该薄膜是在康普茶饮料生产过程中获得的材料的基础上制备的。