The endophyte Burkholderia sp. WYAT7 isolated from the medicinal plant Artemisia nilagirica (Clarke) Pamp. was analyzed for its ability to produce biosurfactant. The evaluation of biosurfactant production was conducted using different screening methods which confirmed the presence of biosurfactant in the culture supernatant. CTAB- methylene blue agar plate method was used for the screening of glycolipid biosurfactant production. The biosurfactant produced by the bacteria effectively metabolized hydrocarbons present in the bacterial culture media. Fourier transform infrared spectroscopic (FTIR) analysis of biosurfactant provided the details regarding O-H stretching, stretching vibrations of acyl chain, C-O stretching, stretching vibrations of ether and vibrations of glycosidic linkages in the biosurfactant. The stretching vibrations of glycosidic linkage in the fingerprint regions of FTIR spectrum (1200 cm^-1 to 800 cm^-1 regions) confirms that the biosurfactant produced was a glycolipid. The GC MS analysis confirmed the methyl and ethyl esters of fatty acids. The biosurfactant from the bacteria exhibited antibacterial activity against bacterial pathogens such as Pseudomonas aeruginosa (MTCC 2453), Escherichia coli (MTCC 1610), Salmonella paratyphi and Bacillus subtilis. The glycolipid biosurfactant had antibiofilm activity as evidenced in Staphylococcus aureus (MTCC 1430). All these results indicated the beneficial effect of the biosurfactant in plant-endophyte interactions. The properties exhibited by the biosurfactant suggest that it can be exploited commercially for the production of novel antibiotics.

内生菌伯克霍尔德氏菌。从药用植物蒿蒿中分离到的WYAT7（克拉克）帮宝适。分析了其产生生物表面活性剂的能力。使用不同的筛选方法进行生物表面活性剂生产的评估，这些方法确认了培养上清液中生物表面活性剂的存在。CTAB-亚甲基蓝琼脂平板法用于筛选糖脂生物表面活性剂的生产。由细菌产生的生物表面活性剂有效地代谢了细菌培养基中存在的碳氢化合物。生物表面活性剂的傅里叶变换红外光谱（FTIR）分析提供了有关生物表面活性剂中OH拉伸，酰基链拉伸振动，CO拉伸，醚拉伸振动和糖苷键振动的详细信息。FTIR光谱（1200 cm ^-1）指纹区糖苷键的拉伸振动到800 cm ^-1区域）确认所产生的生物表面活性剂是糖脂。GC MS分析证实了脂肪酸的甲酯和乙酯。来自细菌的生物表面活性剂对诸如铜绿假单胞菌（MTCC 2453），大肠杆菌（MTCC 1610），副伤寒沙门氏菌和枯草芽孢杆菌等细菌性病原体表现出抗菌活性。糖脂生物表面活性剂具有抗生物膜活性，如金黄色葡萄球菌所示（MTCC 1430）。所有这些结果表明生物表面活性剂在植物与内生菌相互作用中的有益作用。生物表面活性剂表现出的特性表明，它可以被商业开发用于生产新型抗生素。