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Bioactive constituents, vitamin analysis, antioxidant capacity and α-glucosidase inhibition of Canna indica L. rhizome extracts
Food Bioscience ( IF 5.2 ) Pub Date : 2020-02-17 , DOI: 10.1016/j.fbio.2020.100544
S. Ayusman , P. Duraivadivel , H.G. Gowtham , S. Sharma , P. Hariprasad

Rhizomes of Canna indica were studied to determine their potential use as a functional food, a source of vitamins, nutritional and nutraceutical ingredients. Biomass and nutrient characterization showed the rhizomes were a good source of fiber (25.1%), starch (28.5%), crude protein (4.72%), and lipids (5.75%) with a total estimated caloric value of 423 Kcal/100 g dry weight. The rhizome also had considerable amounts of minerals and vitamins. Acetone extracts of rhizomes showed significantly higher antioxidant properties. The IC50 values with DPPH, ABTS+. and O2.- radicals were 21, 23 and 170 μg/ml, respectively. The reducing properties (FRAP and CUPRAC) and DNA protection assay were correlated with the total phenolic and flavonoid content of the rhizome extracts. Acetone and methanol extracts showed protection against free radical-induced DNA and protein degradation. In a β-carotene-linoleic acid model, the acetone extracts significantly decreased the bleaching of β-carotenoids. In a meat model system, the acetone extracts minimized the thiobarbituric acid reactive substance of ground pork meat. α-Glucosidase activity was significantly inhibited using water extracts (IC50 2.35 μg/ml) and acetone extracts (IC50 27.1 μg/ml). HR-LCMS/MS analysis of different extracts showed the occurrence of different bioactive compounds such as rosmarinic acid, psoromic acid, usnic acid, isoeugenitol, ellagic acid, coumaric acid and swietenine. The results suggested that C. indica rhizomes might be a potential source of nutrients and metabolites with health benefits.



中文翻译:

Canna indica L.根茎提取物的生物活性成分,维生素分析,抗氧化能力和α-葡萄糖苷酶抑制作用

印度卡纳的根茎进行了研究,以确定其作为功能食品,维生素,营养和营养成分的来源的潜在用途。生物质和营养素特征表明,根茎是纤维(25.1%),淀粉(28.5%),粗蛋白(4.72%)和脂质(5.75%)的良好来源,总热量估计为423 Kcal / 100 g干重量。根茎还含有大量的矿物质和维生素。根茎的丙酮提取物显示出明显更高的抗氧化性能。IC 50的值为DPPH,ABTS +。和O 2 .-自由基分别为21、23和170μg/ ml。还原特性(FRAP和CUPRAC)和DNA保护测定与根茎提取物中总酚和类黄酮含量相关。丙酮和甲醇提取物对自由基诱导的DNA和蛋白质降解具有保护作用。在β-胡萝卜素-亚油酸模型中,丙酮提取物显着减少了β-类胡萝卜素的漂白。在肉类模型系统中,丙酮提取物可将猪肉末的硫代巴比妥酸反应性物质降至最低。使用水提取物(IC 50 2.35μg/ ml)和丙酮提取物(IC 50)可显着抑制α-葡萄糖苷酶的活性27.1微克/毫升)。不同提取物的HR-LCMS / MS分析表明,存在着不同的生物活性化合物,如迷迭香酸,间苯二酸,松萝酸,异丁香糖醇,鞣花酸,香豆酸和猪藤素。结果表明,印度C假茎的根茎可能是具有健康益处的营养物质和代谢产物的潜在来源。

更新日期:2020-02-17
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