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Obesity, insulin resistance, adiponectin, and PPAR-γ gene expression in broiler chicks fed diets supplemented with fat and green tea (Camellia sinensis) extract.
Domestic Animal Endocrinology ( IF 2.1 ) Pub Date : 2020-01-24 , DOI: 10.1016/j.domaniend.2020.106440
F Mohammadpour 1 , H Darmani-Kuhi 1 , A Mohit 1 , M M Sohani 2
Affiliation  

Adipose tissue is an active endocrine organ secreting several adipokines, especially adiponectin, that play an important role in regulating insulin function in the body of mammals. Therefore, this study was aimed to investigate the association between abdominal fat deposit, insulin resistance, peroxisome proliferator-activated receptor gamma (PPAR-γ), and adiponectin gene (AG) expression in broiler chicks fed diets high in unsaturated fat supplemented with green tea extract (GTE). A total of 300 one-day-old female Ross 308 broiler chicks were allocated to 6 dietary treatments in a completely randomized design with a factorial arrangement of two levels of GTE (0 and 500 mg/kg diet) × three levels of fat inclusion [without fat (control group), soybean oil (SO), and tallow (Ta)]. Each treatment was replicated five times. At the end of the experiment (day 49), two chicks from each replicate weighing an average of pen weight were bled and then slaughtered for further analysis. Abdominal fat percentage, fasting concentration of blood glucose, triglyceride and insulin, glycogen reserves of breast and liver tissues, and PPAR-γ and AG expression were determined. The insulin resistance index of the Quantitative Insulin Sensitivity Check Index (QUICKI) was calculated using the fasting plasma glucose and insulin concentrations. The highest abdominal fat percentage and the lowest carcass yield were obtained in chicks fed SO-supplemented diet (P < 0.05). Chicks fed diet supplemented with SO showed the highest PPAR-γ gene expression (P < 0.05). SO-rich diets suppressed AG expression in chickens' abdominal fat tissue, and the birds fed with SO-supplemented diet showed a significant decrease in AG expression compared with the control (P < 0.05). Chicks fed diet supplemented with SO showed lower QUICKI and breast glycogen reserve compared with the control group (P < 0.05). A significant increase in blood glucose and triglyceride concentrations was observed in birds fed SO-supplemented diets (P < 0.05). AG and PPAR-γ expression increased and decreased by GTE, respectively. QUICKI tended (P = 0.09) to be greater in GTE-supplemented chicks; however, the effect of GTE supplementation on carcass yield, abdominal fat percentage, and blood insulin and glucose concentration was not significant. The findings of this study showed that SO-rich diets via increased PPAR-γ gene expression and decreased AG expression in abdominal fat may lead to insulin resistance in female broiler chicks.



中文翻译:

饲喂添加了脂肪和绿茶(茶花)提取物的肉仔鸡的肥胖,胰岛素抵抗,脂联素和PPAR-γ基因表达。

脂肪组织是一种活跃的内分泌器官,分泌几种脂肪因子,尤其是脂连蛋白,在调节哺乳动物体内的胰岛素功能中起着重要作用。因此,本研究旨在研究饲喂高脂不饱和脂肪补充绿茶日粮的肉鸡的腹部脂肪沉积,胰岛素抵抗,过氧化物酶体增殖物激活受体γ(PPAR-γ)和脂联素基因(AG)表达之间的关系。提取(GTE)。总共300只1日龄雌性Ross 308肉鸡以完全随机的设计分配给6种饮食处理,其中有两个水平的GTE(0和500 mg / kg饮食)×3个脂肪含量水平[不含脂肪(对照组),大豆油(SO)和牛脂(Ta)]。每种处理重复五次。实验结束时(第49天),取每个平均重量为笔重的两只小鸡放血,然后宰杀以进行进一步分析。测定腹部脂肪百分比,血糖,甘油三酸酯和胰岛素的空腹浓度,乳房和肝脏组织的糖原储备以及PPAR-γ和AG表达。使用空腹血浆葡萄糖和胰岛素浓度计算定量胰岛素敏感性检查指数(QUICKI)的胰岛素抵抗指数。饲喂含SO饲料的雏鸡的腹部脂肪最高,car体产量最低(测定乳腺和肝组织的糖原储备,PPAR-γ和AG表达。使用空腹血浆葡萄糖和胰岛素浓度计算定量胰岛素敏感性检查指数(QUICKI)的胰岛素抵抗指数。饲喂含SO饲料的雏鸡的腹部脂肪最高,car体产量最低(测定乳腺和肝组织的糖原储备,PPAR-γ和AG表达。使用空腹血浆葡萄糖和胰岛素浓度计算定量胰岛素敏感性检查指数(QUICKI)的胰岛素抵抗指数。饲喂含SO饲料的雏鸡的腹部脂肪最高,car体产量最低(P  <0.05)。补充SO的雏鸡的PPAR-γ基因表达最高(P <0.05)。富含SO的饮食抑制了鸡腹部脂肪组织中的AG表达,饲喂添加了SO的饮食的鸟类与对照组相比,AG的表达显着下降(P <0.05)。与对照组相比,补充了SO的雏鸡的QUICKI和乳房糖原储备较低(P <0.05)。在饲喂含SO饲料的禽类中,观察到血糖和甘油三酸酯浓度显着增加(P  <0.05)。GTE使AG和PPAR-γ表达分别升高和降低。QUICKI趋向于(P= 0.09)在补充GTE的雏鸡中更大; 然而,补充GTE对on体产量,腹部脂肪百分比以及血液中胰岛素和葡萄糖浓度的影响并不显着。这项研究的结果表明,富含SO的饮食通过增加PPAR-γ基因的表达和降低腹部脂肪中AG的表达可能导致雌性肉鸡雏鸡胰岛素抵抗。

更新日期:2020-01-24
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